<ORIGINAL>Quantification of Porphyromonas gingivalis by real time PCR : new primers targeting the rgpA and rgpB gene encoding RGP
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概要
- 論文の詳細を見る
We designed new primers for the quantification of Porphyromonas gingivalis by real time PCR. The new primer set targeted the rgpA and rgpB genes that encode arginine specific cysteine proteinase (Arggingipain or Rgp), one of the putative pathogenic factors of P. gingivalis. The PCR product obtained using our primers showed no by-products by melting curve analysis. The PCR product sequence showed no significant matches to other sequences by BLAST searching of genetic databases except for matches to P. gingivalis rgpA and rgpB sequence, and could not be amplified from template derived from other oral bacteria apart from P. gingivalis. Therefore, we concluded that our primers were specific for P. gingivalis rgpA and rgpB, and could be used to quantity from 10^3 to 10^7 P. gingivalis cells when applied to real time PCR.
- 北海道医療大学の論文
- 2003-06-30
著者
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WATANABE Toshihiro
Department of Food Science and Technology, Tokyo University of Agriculture
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Ohyama Tohru
Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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Ohyama T
Department Of Food Sciences And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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NAKAMURA Reiko
Department of Orthodontics and Dentofacial Orthopedics, Osaka University Faculty of Dentistry
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KAMAGUCHI Arihide
Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido
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Watanabe Toshihiro
Department Of Food Sciences And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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Watanabe Toshihiro
Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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Baba Hisae
Deoartment Of Oral Microbiology School Of Dentistry Health Sciences University Of Hokkaido
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Nakamura Reiko
Department Of Oral Microbiology School Of Dentistry Health Sciences University Of Hokkaido
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Nakamura Reiko
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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OKAMOTO Akimasa
Department of Oral Bacteriology, School of Dental Medicine, Turumi University
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Kamaguchi Arihide
Department Of Oral Microbiology School Of Dentistry Health Sciences University Of Hokkaido
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Okamoto Akimasa
Department Of Oral Bacteriology School Of Dental Medicine Turumi University
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Watanabe Toshihiro
Department Of Applied Mathematics University Of Gifu
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KAMAGUCHI Arihide/NAKAMURA
Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido
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