The Effect of a Shear Flow on the Uptake of LDL and Ac-LDL by Cultured Vascular Endothelial Cells(<Special Issue>Bioengineering)

スポンサーリンク

概要

• 論文の詳細を見る

• The effects of a shear flow on the uptake of fluorescence-labeled low-density lipoprotein (DiI-LDL), acetylated LDL (DiI-Ac-LDL), and lucifer yellow (LY; a tracer of fluid-phase endocytosis) by cultured bovine aortic ECs were studied using a rotating-disk shearing apparatus. It was found that 2 hours' exposure of ECs to a laminar shear flow that imposed ECs an area-mean shear stress of 10 dynes/cm^2 caused an increase in the uptake of DiI-LDL and LY. By contrast, the uptake of DiI-Ac-LDL was decreased by exposure of the ECs to a shear flow. Addition of dextran sulfate (DS), a competitive inhibitor of scavenger receptors, reversed the effect of a shear flow on the uptake of DiI-Ac-LDL, resulting in an increase by the imposition of a shear flow, while the uptake of DiI-LDL and LY remained unaffected. It was concluded that a shear flow promotes the endocytosis of DiI-LDL and LY by ECs, but suppresses the uptake of DiI-Ac-LDL by ECs by inhibiting scavenger receptor-mediated endocytosis.

• 一般社団法人日本機械学会の論文
• 2005-12-15

著者

• Karino Takeshi

  Laboratory Of Biofluid Dynamics Research Institute For Electronic Science Hokkaido University

• Niwa Koichi

  Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture

• Karino Takeshi

  Laboratory of Biofluid Dynamics, Research Institute for Electronic Science, Hokkaido University

• NIWA Koichi

  Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099–2493, Japan

関連論文

• Involvement of Sialic Acid in Transport of Serotype C1 Botulinum Toxins through Rat Intestinal Epithelial Cells
• The Effect of a Shear Flow on the Uptake of LDL and Ac-LDL by Cultured Vascular Endothelial Cells(Bioengineering)
• The Effects of a Shear Flow and Water Filtration on the Structure of Cell Layers in a Hybrid Vascular Graft : An In Vitro Study(Micro- and Nano-biomechanics)
• IONIC BASIS OF SECRETORY RESPONSE TO PHYSIOLOGICAL CONCENTRATION OF CHOLECYSTOKININ OCTAPEPTIDE IN ISOLATED PERFUSED RAT PANCREAS
• Botulinum Toxin Complex Increases Paracellular Permeability in Intestinal Epithelial Cells via Activation of p38 Mitogen-Activated Protein Kinase

スポンサーリンク