Expression Pattern of a Chloroplast NADPH-Dependent Thioredoxin Reductase in Chlorella vulgaris during Hardening and Its Interaction with 2-Cys Peroxiredoxin
スポンサーリンク
概要
- 論文の詳細を見る
A chloroplastic NADPH-dependent thioredoxin reductase gene was identified from Chlorella vulgaris and designated CvNTRC. Mature CvNTRC protein (mCvNTRC) was expressed in Escherichia coli, and it showed both NADPH-dependent thioredoxin reductase (NTR) and thioredoxin (Trx)-like dithiol-disulfide oxidoreductase activities. The transcript of CvNTRC increased throughout 24-h hardening, whereas the encoded protein amount and total NTR activity decreased once and then increased during hardening. By in vitro pull-down assay, a 21.2-kDa protein bound to mCvNTRC was isolated and identified as a 2-Cys peroxiredoxin (2-Cys Prx) based on the N-terminal sequence. These data suggest that CvNTRC is maintained at a constant level during hardening and functions as an antioxidant with 2-Cys Prx in the acquisition of freezing tolerance of Chlorella.
- 社団法人 日本農芸化学会の論文
著者
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MIYAMOTO Takahisa
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu Universi
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MACHIDA Takeshi
Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Scie
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Honjoh Ken-ichi
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Kyushu University
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Machida Takeshi
Kyushu Univ. Fukuoka Jpn
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NIIMURA Youichi
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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KATO Eri
Department of Earth and Space Science, Graduate School of Science, Osaka University
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KAWASAKI Shinji
Department of Bioscience, Tokyo University of Agriculture
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SATO Jun-ichi
Department of Physics, School of Science, Tokai University
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Ishibashi Akiko
Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sci
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