Inhibitory Monoclonal Antibody against Japanese Radish Acid Phosphatase
スポンサーリンク
概要
- 論文の詳細を見る
Thirty stable hybridoma cell lines secreting monoclonal antibodies specific for Japanese radish acid phosphatase (EC 3.1.3.2) were obtained. These antibodies were inhibitory or stimulatory or had no effect on the enzyme activity. Four antibodies (IgG_1 subclass) among them, designated MAb-11, MAb-18, MAb-20, and MAb-30, were purified and partially characterized. MAb-11, MAb-18, and MAb-20 inhibited more than 80% of the enzyme activity and appeared to act as noncompetitive inhibitors. Competitive inhibition assay indicated that MAb-18 and MAb-20 were classified into the same group and MAb-11 into another group. MAb-20 had an inhibitory effect on acid phosphatases from some vegetable and meat sources. The Fab fragment prepared by limited proteolysis of MAb-20 with papain also inhibited the enzyme.
- 社団法人日本農芸化学会の論文
- 1993-11-23
著者
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Sakamoto J
Shimane Univ. Matsue Jpn
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宮本 敬久
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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MIYAMOTO Takahisa
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu Universi
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HATANO Shoji
Graduate School of Health and Social Welfare Science, Nishikyushu University
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YOSHIMOTO Makoto
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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Sakamoto J
Food Research Laboratories Ajinomoto Co. Inc.
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TAKATA Ryoji
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, G
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SOEDA Takahiko
Food Research Laboratories, Ajinomoto Co. Inc.
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SAKAMOTO Jiro
Food Research Laboratories, Ajinomoto Co. Inc.
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HATANO Shoji
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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Yoshimoto Makoto
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Hatano Shoji
Department Of Food Science And Technology Faculty Of Agriculture 46-09 Kyushu University
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Takata Ryoji
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Soeda Takahiko
Food Research Laboratories Ajinomoto Co. Inc.
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Hatano S
Kyushu Univ. Fukuoka Jpn
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Miyamoto T
Kyushu Univ. Fukuoka Jpn
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Tamai Rumiko
Laboratory Of Food Hygienic Chemistry Faculty Of Agriculture Kyushu University
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Yoshimoto M
Sweetpotato Breeding Research Team National Agricultural Research Center For Kyushu Okinawa Region
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SAKAMOTO Jiro
Department of Physics, Shimane University
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SAKAMOTO Jiro
Physics Department, Shimane University
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