Modification of Lipases with Poly(Ethylene Glycol)and Poly(Oxyethylene)Detergents and Their Catalytic Activities in Organic Solvents
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概要
- 論文の詳細を見る
The α-chymotrypsin-poly(ethylene glycol)complex, which was prepared by lyophilizing an aqueous solution, was found to have high catalytic activity in organic media even when the molar ratio of polymer/enzyme in its preparation stage is unity. In this study, we obtained freeze-dried complexes of lipases and poly(ethylene glycol)or poly(oxyethylene)detergents including newly synthesized gemini-type detergents, and their transesterification activity in organic solvents was evaluated. The freeze-dried lipase from Pseudomonas cepacia prepared by using each modifier showed enhanced transesterification activity, exhibiting a similar dependence on the concentration of the modifier in the preparation stage to that of the α-chymotrypsin-poly(ethylene glycol)complex ; in contrasts, the one from Candida rugosa did not do so.
- 社団法人日本生物工学会の論文
- 2001-12-25
著者
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NAKAO Katsumi
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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FURUMOTO Keiji
Oshima National College of Maritime Technology
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YOSHIMOTO Makoto
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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MINE YURIE
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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FUKUNAGA KIMITOSHI
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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SUGIMURA YOSHIAKI
General Education Division, Oshima National College of Maritime Technology
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Yoshimoto Makoto
Department Of Food Science And Technology Faculty Of Agriculture Kyushu University
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Yoshimoto Makoto
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Yoshimoto M
Department Of Upland Farming Research National Agricultural Research Center For Kyushu Okinawa Regio
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Mine Yurie
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Nakao Katsumi
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Yoshimoto M
Sweetpotato Breeding Research Team National Agricultural Research Center For Kyushu Okinawa Region
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Fukunaga K
Oshima National College Of Maritime Technology
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Sugimura Yoshiaki
General Education Division Oshima National College Of Maritime Technology
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Fukunaga Kimitoshi
Department Of Applied Chemistry And Chemical Engineering Yamaguchi University
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