Preparation of Detergent-Lipase Complexes Utilizing Water-Soluble Amphiphiles in Single Aqueous Phase and Catalysis of Transesterifications in Homogeneous Organic Solvents
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概要
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A novel method of preparing detergent-enzyme complexes that can be employed in organic media was developed utilizing newly synthesized water-soluble nonionic gemini-type detergents, N, N-bis(3-D-gluconamidoplopyl)-3-(dialkyl-L-glutamatecarbonyl)propanamides (BIG2GnCA: n=10, 12, 14, 16, 18) and N, N-bis(3-D-lactonamidopropyl)-3-(dialkyl-L-glutamatecarbonyl)propanamides (BIL2CnCA: n=16, 18), and nonionic twin-headed detergents, N, N-bis(3-D-gluconamidopropyl)alkanamides (BIG1Cn: n=12, 14, 16, 18, Δ^9). This method simply entails mixing a selected enzyme with an appropriate detergent in an aqueous solution followed by lyophilization, and it offers the advantages of enhanced enzymatic activity in organic solvents and eliminated both enzyme loss and the necessity for an organic solvent in the preparation stage. Using various modified lipases originating from Aspergillus niger(Lipase A), Candida rugosa(Lipase C), Pseudomonas cepacia(Lipase P), and porcine pancreas(PPL), prepared using the novel method and detergents, including conventional synthesized nonionic detergents such as dialkyl N-D-glucona-L-glutamates(2CnGE: n=12, 18Δ^9)) and octanoyl-N-methylglucamide(MEGA-8), enantioselective transesterifications of 6-methyl-5-hepten-2-ol(sulcatol) and 2, 2-dimethyl-1, 3-dioxolane-4-methanol(solketal) with a vinyl or isopropenyl carboxylate were carried out in an organic solvent. The modified lipase activity was influenced by both the lipases and the structure of the detergents. The value for the hydrophile-lipophile balance(HLB) of the detergent provided a means of correlating the structure and the obtained modified lipase activity. For detergents of the same class with a HLB value of approximately 9 and 12, the highest activity was obtained for Lipase A and Lipase P, and Lipase C and PPL, respectively. Among detergents of the same HLB value tested, the gemini-type detergents possessing the most bulky head and tail were most effective as a modifier for lipases of all types. The preparation and reaction conditions for these novel gemini-type detergent-modified lipases were optimized using BIG2C_<12>CA(HLB=9.4) by studying the effect of the detergent/lipase ratio and the nature of organic solvents on the complex formation. The high enzymatic activities of the BIG2C_<12>CA-modified lipases were independent of the solubility of the lipases in organic solvents, unlike in the case of 2CnGE-modified lipases prepared using the conventional syspension system.
- 社団法人日本生物工学会の論文
- 2000-12-25
著者
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NAKAO Katsumi
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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FURUMOTO Keiji
Oshima National College of Maritime Technology
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MINE YURIE
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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FUKUNAGA KIMITOSHI
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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SUGIMURA YOSHIAKI
General Education Division, Oshima National College of Maritime Technology
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Mine Yurie
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Nakao Katsumi
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Fukunaga K
Oshima National College Of Maritime Technology
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MARUOKA NOBUAKI
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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Maruoka Nobuaki
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Sugimura Yoshiaki
General Education Division Oshima National College Of Maritime Technology
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Fukunaga Kimitoshi
Department Of Applied Chemistry And Chemical Engineering Yamaguchi University
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