Structural Effects of Amphiphiles on Candida rugosa Lipase Activation by Freeze-Drying of Aqueous Solution of Enzyme and Amphiphile(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
Lipases co-lyophilized with water-soluble gemini-type amphiphiles were found to have high enzyme activity in nonaqueous media without washing out of the amphiphile with anhydrous organic solvent. In this study, we obtained freeze-dried complexes of Candida rugosa lipase (CRL) with six water-soluble twin glusitol-headed amphiphiles bearing different types of hydrophobic tails, including newly synthesized ones, and their transesterification activity in organic solvent was evaluated. The results indicate that the increased enzyme activity upon CRL modification at 200 molar ratio of amphiphile/CRL, which are restricted to the ester-containing amphiphiles, is probably due to the surface activation by the interaction between ester-carbonyl of the amphiphile and phenyl group of the tyrosine residue situated on the surface of the lid in the CRL.
- 社団法人日本生物工学会の論文
- 2003-12-25
著者
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Samejima Ken-ichi
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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NAKAO Katsumi
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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FURUMOTO Keiji
Oshima National College of Maritime Technology
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YOSHIMOTO Makoto
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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MINE YURIE
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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FUKUNAGA KIMITOSHI
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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SUGIMURA YOSHIAKI
General Education Division, Oshima National College of Maritime Technology
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Yoshimoto Makoto
Department Of Food Science And Technology Faculty Of Agriculture Kyushu University
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Yoshimoto Makoto
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Yoshimoto M
Department Of Upland Farming Research National Agricultural Research Center For Kyushu Okinawa Regio
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Mine Yurie
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Nakao Katsumi
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Yoshimoto M
Sweetpotato Breeding Research Team National Agricultural Research Center For Kyushu Okinawa Region
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Fukunaga K
Oshima National College Of Maritime Technology
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Sugimura Yoshiaki
General Education Division Oshima National College Of Maritime Technology
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Fukunaga Kimitoshi
Department Of Applied Chemistry And Chemical Engineering Yamaguchi University
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