Purification and Some Properties of an Antibacterial Factor Derived from Bacillus subtilis FHC 402(Biological Chemistry)
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概要
- 論文の詳細を見る
Bacillus subtilis FHC 402-derived antibacterial factor (abbreviated to BAF), which showed antibacterial activity by its combined use with hexametaphosphate, was purified from a culture broth by means of column chromatography on octyl-Sepharose CL-4B, DEAE-Cellulofine AL and Bio-Gel P-6. BAF was purified 63-fold with an activity yield of 17%. The purified preparation gave a single band on the electrophoregram. BAF may be a glycopeptide containing 22 molecules of 8 different amino acids, 3 hexoses, 1 hexosamine, 1 deoxyribose, and unknown substances. The N-terminal amino acid of the BAF preparation was determined to be tyrosine and the C-terminal amino acid to be proline. Gel filtration estimated the molecular weight of the BAF preparation at 3,200. The purified BAF preparation was stable in a pH range from 5.0 to 11.0, was also stable on heating, but was unstable at pH 3.0.
- 社団法人日本農芸化学会の論文
- 1986-05-23
著者
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Yamada Koji
Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu University
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宮本 敬久
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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MIYAMOTO Takahisa
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu Universi
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YOSHIMOTO Makoto
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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TSUTSUMI MASAKAZU
Department of Urology, Hitachi General Hospital
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HATANO Shoji
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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Yoshimoto Makoto
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Tsutsumi Masakazu
Department Of Food Science And Technology Faculty Of Agriculture Kyushu University:(present Office)d
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Yamada K
Ajinomoto Co. Ltd. Kanagawa Jpn
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Hatano Shoji
Department Of Food Science And Technology Faculty Of Agriculture 46-09 Kyushu University
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Hatano S
Kyushu Univ. Fukuoka Jpn
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Yoshimoto M
Sweetpotato Breeding Research Team National Agricultural Research Center For Kyushu Okinawa Region
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Yamada Koji
Deparment Of Food Science And Technology Faculty Of Agriculture Kyushu University
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