Simple High-yield Purification of 3,4,5,-Tri-O-caffeoylquinic Acid from Sweetpotato (Ipomoea batatas L.) Leaf and Its Inhibitory Effects on Aldose Reductase
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概要
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A simple high-yield method for purifying 3,4,5-tri-O-caffeoylquinic acid from sweetpotato leaves (Ipomoea batatas L.) was established. The method consisted of methanol extraction, water-hexane partition, MCI gel CHP20P, and Sephadex LH-20 chromatography. All purification steps required fewer than 3 days, and the yield of purified 3,4,5-tri-O-caffeoylquinic acid was calculated to exceed 76 mg from 100 g of leaf powder. Its recovery was estimated to be more than 66%, and its purity more than 98%. The aldose reductase inhibitory activities of these derivatives were 3,4,5-tri-O-caffeoylquinic acid > 3,4-di-O-caffeoylquinic acid = 3,5-di-O-caffeoylquinic acid > 4,5-di-O-caffeoylquinic acid > 5-O-caffeoylquinic acid. These results indicate that 3,4,5-tri-O-caffeoylquinic acid is the more bioactive component, and this purification method enables in vivo experiments.
- 2011-03-01
著者
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Yamakawa Osamu
Department Of Upland Farming Research National Agricultural Research Center For Kyushu Okinawa Regio
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Yamakawa Osamu
Department Of Internal Medicine Cancer Research Institute Kanazawa University
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Yamakawa Osamu
Department Of Upland Farming Kyusyu National Agricultural Experiment Station
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Yoshimoto Makoto
Department Of Food Science And Technology Faculty Of Agriculture Kyushu University
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Yoshimoto Makoto
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Yahara S
Kumamoto Univ. Kumamoto Jpn
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Yahara Shoji
Graduate School Of Pharmaceutical Science Kumamoto University
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Yoshimoto M
Sweetpotato Breeding Research Team National Agricultural Research Center For Kyushu Okinawa Region
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KURATA Rie
Research Team for Biomass Recycling System, National Agricultural Research Center for Kyushu Okinawa
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YAMAKAWA Osamu
Research Team for Biomass Recycling System, National Agricultural Research Center for Kyushu Okinawa
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YOSHIMOTO Makoto
Research Team for Biomass Recycling System, National Agricultural Research Center for Kyushu Okinawa
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Kurata Rie
Research Team For Biomass Recycling System National Agricultural Research Center For Kyushu Okinawa
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