Oxidative Refolding of Lysozyme Assisted by Negatively Charged Liposomes : Relationship with Lysozyme-Mediated Fusion of Liposomes
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概要
- 論文の詳細を見る
Oxidative refolding of denatured/reduced lysozyme was examined in the presence of charged liposomes composed of neutral 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine(POPC) and negatively charged 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol(POPG). Surface charge density of liposmes had a marked effect on the interaction between liposomes and reduced lysozyme which is observed in the early stage of the refolding. It was found that there was a critical level of surface charge density of liposomes(-0.15C/nm^2) at which the interaction between liposomes and lysozyme drastically changed. At the surface charge density of liposomes ranging from -0.15 to -1.4C/nm^2, the literaction between liposomes and lysozyme resulted in aggregate formation. In contrast, at the surface charge density ranging from 0 to -0.15C/nm^2, no aggregate formation was observed if the lysozyme/liposome molar ratio was less than 600. On the basis of the experimental results, a model for the interaction between charged liposomes and lysozyme was proposed, focusing on the mechanism of protein-induced fusion of charged liposomes as well as protein refolding on liposomes. Then, the optimal condition for oxidative refolding of lysozyme was examined in the presence of charged liposomes by controlling the lysozyme-liposome interaction. The reactivation yield of lysozyme was improved up to 85% in the presence of liposomes with a surface charge density of -0.14C/nm^2.
- 社団法人日本生物工学会の論文
- 2000-07-25
著者
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Kuboi Ryoichi
Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka Univer
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Kuboi Ryoichi
Department Of Chemical Engineering Osaka University
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YOSHIMOTO Makoto
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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Yoshimoto Makoto
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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MAWATARI TAKESHI
Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka Univer
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Kuboi Ryoichi
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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Yoshimoto Makoto
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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Mawatari Takeshi
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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Mawatari Takeshi
Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture
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