Control of Partitioning of Bacterial Cells and Characterization of Their Surface Properties in Aqueous Two-Phase Systems
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概要
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A systematic approach to the characterization of the physicochemical properties of bacterial cell surfaces (isoelectric point, surface net and local hydrophobicity) has been presented using the same aqueous two-phase partitioning method that was applied for the characterization of protein surfaces (Kuboi et al., Solv. Extr. Res. Dev. Japan, 1,42-52,1995). It was found that the isoelectric point (pI) could be determined by cross partition method and that the pI of Escherichia coli cells is generally low. At the pI, the surface hydrophobicity (HFS) of the bacterial cells was determined by using the slope of the relationship between the hydrophobic differences of the two phases and the partition coefficient of the bacterial cells. The partition behavior of cells was found to be influenced by the addition of relatively low concentration of salts. The addition of nonionic surfactants of the Triton X-series led to an increase in the partitioning of the cells (Δln K_<Cell>) by causing a hydrophobic interaction between Triton molecules and the cell surface. This effect is probably due to the interaction between the hydrophobic groups of the surfactant and the local hydrophobic binding sites of the cell surface, such as the membrane proteins and phospholipid membranes. By using these parameters, such as the pI, HFS, and Δln K_<Cell>, the surface properties of bacterial cells which were exposed by heat treatment were analyzed.
- 社団法人日本生物工学会の論文
- 1997-12-25
著者
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Kuboi Ryoichi
Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka Univer
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Kuboi Ryoichi
Osaka University
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Umakoshi Hiroshi
Department Of Chemical Science And Engineering Graduated School Of Engineering Science Osaka Univers
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Kuboi R
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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Kuboi Ryoichi
Department Of Chemical Engineering Osaka University
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駒沢 勲
Department Of Chemical Science And Engineering Graduate School Of Engineering Science And Research C
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Komasawa Isao
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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Komasawa Isao
Department Of Chemical Engineering Faculty Of Engineering Science Osaka University
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UMAKOSHI HITOSHI
Department of Chemical Science and Engineering, Graduated School of Engineering Science, Osaka Unive
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Umakoshi Hiroshi
Department Of Chemical Engineering Osaka University
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Umakoshi Hitoshi
Department Of Chemical Science And Engineering Graduated School Of Engineering Science Osaka Univers
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