Effect of Electrostatic Interaction on Reverse Micellar Extraction of Large Molecular Weight Proteins
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概要
- 論文の詳細を見る
Reverse micellar extraction of various proteins of large molecular weight and oligomeric proteins, as well as small ones, was carried out using conventional AOT reverse micellar extraction systems at various pH values. The conformational change of the proteins by electrostatic interaction with AOT was also measured using a CD spectrum. Extraction behavior and conformational change of the proteins were discussed from their electric charge properties and electrostatic interaction between protein and AOT. Most of the proteins were effectively extracted into the reverse micellar phase at around zero surface net charge. Formation of aggregates and denaturation of proteins were observed in the region of positive net charge higher than 10.
- 社団法人 化学工学会の論文
著者
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塩盛 弘一郎
宮崎大学工学部 物質環境科学科
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Shiomori Koichiro
Department of Material Environment Chemistry, Faculty of Engineering, Miyazaki University
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Kuboi Ryoichi
Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka Univer
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Kuboi Ryoichi
Osaka University
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Kuboi R
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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Kuboi Ryoichi
Department Of Chemical Engineering Osaka University
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Komasawa Isao
Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka Univer
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河野 恵宣
宮崎大学名誉教授
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駒沢 勲
Department Of Chemical Science And Engineering Graduate School Of Engineering Science And Research C
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Komasawa Isao
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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KAWANO Yoshinobu
Department of Applied Chemistry, Miyazaki University
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Komasawa Isao
Department Of Chemical Engineering Faculty Of Engineering Science Osaka University
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河野 恵宣
宮崎大学工学部
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Shiomori Koichiro
Department Of Applied Chemistry Miyazaki University
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Kawano Yoshinobu
Department Of Applied Chemistry Miyazaki University
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Kawano Y
Miyazaki Univ. Miyazaki
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