Control of Autolysis of Bacillus subtilis for Selective Production of the Intracellular Enzyme Glucose-6-Phosphate Dehydrogenase in Aqueous Two-Phase Systems
スポンサーリンク
概要
- 論文の詳細を見る
A method for the release of intracellular enzyme by autolysis of Bacillus subtilis cells is presented. Both the growth and lysis processes were further applied to aqueous two-phase systems (ATPS). Lysis induced by the addition of Triton X-100 and by low-temperature treatment facilitated the release of cytoplasmic enzyme glucose-6-phosphate dehydrogenase (G6PDH) in ATPS. The release selectivity increased when lysis was regulated by the addition of 50 μM or 100 μM Triton X-100. Cardiolipin efficiently inhibited the autolytic process. Control of the autolytic system promoted the selective release of G6PDH. B. subtilis cells could be grown and lysed in aqueous two-phase systems in a similar fashion to the conventional single-phase medium solutions. The released enzymes were partitioned according to their surface properties. G6PDH were extracted to the top phase in a PEG1540/Dex100K-200K system. Cells were partitioned to the bottom phase or the interface, and could be recycled into the fermentor. The selectivity of enzyme production was also increased in two-phase system by the addition of cardiolipin.
- 社団法人日本生物工学会の論文
- 1993-06-25
著者
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Kuboi Ryoichi
Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka Univer
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Kuboi Ryoichi
Osaka University
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Kuboi R
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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Kuboi R
Department Of Chemical Engineering Osaka University
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Kuboi Ryoichi
Department Of Chemical Engineering Osaka University
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駒沢 勲
Department Of Chemical Science And Engineering Graduate School Of Engineering Science And Research C
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Komasawa Isao
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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Komasawa Isao
Department Of Chemical Engineering Faculty Of Engineering Science Osaka University
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TANAKA HISAKAZU
Department of Chemical Engineering, Osaka University
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TSUCHIDO TETSUAKI
Department of Biotechnology, Kansai University
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Tanaka H
Institute Of Applied Biochemistry University Of Tsukuba
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Tsuchido T
Department Of Biotechnology Faculty Of Engineering Kansai University
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Tsuchido Tetsuaki
Department Of Biotechnology Faculty Of Engineering Kansai University
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Tsuchido Tetsuaki
Department Of Biotechnology Faculty Of Engineering And High Technology Research Center Kansai Univer
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