Simultaneous Enrichment of Salmonella spp, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Bacillus cereus, and Listeria monocytogenes by Single Broth and Screening of the Pathogens by Multiplex Real-time PCR
スポンサーリンク
概要
- 論文の詳細を見る
Simultaneous enrichment broth (SEB) was developed for the single-enrichment simultaneous screening of six major food poisoning bacteria. After enrichment in SEB for 18 h at 37°C, viable counts of six major food poisoning bacteria (Staphylococcus aureus, Salmonella spp., Escherichia coli O157:H7, Vibrio parahaemolyticus, Bacillus cereus, and Listeria monocytogenes) were sufficient for 5 nuclease multiplex real-time PCR assay using existing primers and probes. By labeling the probes with three different fluorescent dyes, the assay could be carried out in 2 tubes. The whole process, including enrichment and PCR, was completed within 24 h and the detection limits for the target bacteria from the food sample (boiled chicken) were 36 cfu/25 g for S. aureus, 5.3 cfu/25 g for Salmonella spp, 2.9 cfu/25 g for E. coli O157:H7, 2.0 cfu/25 g for V. parahaemolyticus, 5.5 cfu/25 g for B. cereus, and 6.2 cfu/25 g for L. monocytogenes. The results were comparable to conventional methods that require 4-6 days.
- 2009-07-01
著者
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KOBAYASHI Hiroshi
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and
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KUBOTA Jun
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and
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FUJIHARA Kumiko
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and
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HONJOH Ken-ichi
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and
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IIO Masayoshi
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and
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FUJIKI Naomi
Japan Food Research Laboratories, Kyushu Branch
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NAKABE Mika
Japan Food Research Laboratories, Kyushu Branch
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ODA Shun-ichi
Japan Food Research Laboratories, Kyushu Branch
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SATOYAMA Toshiya
Japan Food Research Laboratories, Kyushu Branch
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TAKASU Kazushige
Japan Food Research Laboratories, Osaka Branch
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NAKANISHI Hisao
Japan Food Research Laboratories, Osaka Branch
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MIYAMOTO Takahisa
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and
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宮本 敬久
九州大学大学院農学研究院生物機能科学部門食品バイオ工学講座食品衛生化学分野
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宮本 敬久
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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Iio Masayoshi
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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Honjoh K
Kyushu Univ. Fukuoka Jpn
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Honjoh Ken-ichi
Laboratory Of Food Hygienic Chemistry Department Ofbioscience And Biotechnology Faculty Of Agricultu
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Honjoh Ken-ichi
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Kyushu University
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Machida Takeshi
Kyushu Univ. Fukuoka Jpn
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Sakai Takatoshi
Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu Unviersity
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Sakai Takatoshi
Japan Food Research Laboratories Kyushu Branch
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Miyamoto Takahisa
Laboratory Of Food Hygienic Chemistry Faculty Of Agriculture Kyushu University
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Fujihara Kumiko
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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Miyamoto T
Kyushu Univ. Fukuoka Jpn
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Nakayama Motokazu
Japan Food Research Laboratories Kyushu Branch
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Nakanishi Hisao
Japan Food Research Laboratories Osaka Branch
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Takasu Kazushige
Japan Food Research Laboratories Osaka Branch
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Fujiki Naomi
Japan Food Research Laboratories Kyushu Branch
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Kubota Jun
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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Oda Shun-ichi
Japan Food Research Laboratories Kyushu Branch
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Kobayashi Hiroshi
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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Nakabe Mika
Japan Food Res. Lab. Fukuoka Jpn
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