Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27
スポンサーリンク
概要
- 論文の詳細を見る
Protoplasts from Chlorella vulgaris K-73112 were obtained by enzymatic digestion with a mixture of Acromopeptidase, Cellulase ONOZUKA R-10, Chitosanas Kl, Gluczyme, and Uskizyme. The formation of naked protoplasts was confirmed by fluorescence microscopy using fluorescent brightner 28, which stains cell walls. About 88% of C. vulgaris K-73112 cells were covereted into osmotically-labile cells. Futhermore, a method for regeneration of intact cells from the protoplasts was developed. Utilization of 0.5M sucrose as an osmoticum, Fe-EDITA as an iron source, and bacto-agar as a supporting was shown to help regeneration of the cell walls of two strains, C. vulgaris K-73112 and C-27.
- 九州大学の論文
著者
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HONJOH Ken-ichi
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and
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Honjoh K
Kyushu Univ. Fukuoka Jpn
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Honjoh Ken-ichi
Laboratory Of Food Hygienic Chemistry Department Ofbioscience And Biotechnology Faculty Of Agricultu
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Honjoh Ken-ichi
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Kyushu University
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Suga Koushirou
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and
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Shinohara Fuminori
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and
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Suga Koushirou
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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Sadakari Kiyoko
Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu University
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Sukimoto Kouji
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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Shinohara Fuminori
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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Shinohara Fuminori[et
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
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