Expression Pattern of a Chloroplast NADPH-Dependent Thioredoxin Reductase in Chlorella vulgaris during Hardening and Its Interaction with 2-Cys Peroxiredoxin
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概要
- 論文の詳細を見る
A chloroplastic NADPH-dependent thioredoxin reductase gene was identified from Chlorella vulgaris and designated CvNTRC. Mature CvNTRC protein (mCvNTRC) was expressed in Escherichia coli, and it showed both NADPH-dependent thioredoxin reductase (NTR) and thioredoxin (Trx)-like dithiol-disulfide oxidoreductase activities. The transcript of CvNTRC increased throughout 24-h hardening, whereas the encoded protein amount and total NTR activity decreased once and then increased during hardening. By in vitro pull-down assay, a 21.2-kDa protein bound to mCvNTRC was isolated and identified as a 2-Cys peroxiredoxin (2-Cys Prx) based on the N-terminal sequence. These data suggest that CvNTRC is maintained at a constant level during hardening and functions as an antioxidant with 2-Cys Prx in the acquisition of freezing tolerance of Chlorella.
- 社団法人 日本農芸化学会の論文
- 2009-03-23
著者
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宮本 敬久
九州大学大学院農学研究院生物機能科学部門食品バイオ工学講座食品衛生化学分野
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宮本 敬久
Laboratory Of Food Hygienic Chemistry Division Of Food Biotechnology Department Of Bioscience And Bi
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MIYAMOTO Takahisa
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu Universi
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MACHIDA Takeshi
Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Scie
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Honjoh K
Kyushu Univ. Fukuoka Jpn
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Honjoh Ken-ichi
Laboratory Of Food Hygienic Chemistry Department Ofbioscience And Biotechnology Faculty Of Agricultu
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Honjoh Ken-ichi
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Kyushu University
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Machida Takeshi
Kyushu Univ. Fukuoka Jpn
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NIIMURA Youichi
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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KATO Eri
Department of Earth and Space Science, Graduate School of Science, Osaka University
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KAWASAKI Shinji
Department of Bioscience, Tokyo University of Agriculture
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SATO Jun-ichi
Department of Physics, School of Science, Tokai University
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Machida Takeshi
Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien
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Ishibashi Akiko
Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sci
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Niimura Youichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Niimura Youichi
Department Of Bioscience Tokyo University Of Agriculture
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Miyamoto T
Kyushu Univ. Fukuoka Jpn
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Ishibashi Akiko
Department Of Bioscience And Biotechnology Graduate School Of Bioresources And Bioenvironmental Scie
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Kato Eri
Department Of Bioscience And Biotechnology Graduate School Of Bioresources And Bioenvironmental Scie
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Kawasaki Shinji
Department Of Bioscience Tokyo University Of Agriculture
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Sato Jun-ichi
Department Of Bioscience Tokyo University Of Agriculture
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Sato Jun-ichi
Department Of Applied Fine Chemistry Faculty Of Engineering Osaka University
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