A Surface Plasmon Resonance-based Immunosensor for Sensitive Detection of Bisphenol A

概要

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A method for the determination of bisphenol A (BPA), a representative endocrine-disrupting chamicals, was developed usnig the surface plasmon resonance (SPR) sensor. BPA-ovalbumin (BPA-OVA) conjugate was immobolized on an Au thin film of the SPR sensor chip by physixal absorption, and BPA determinations were performed by an indirect competitive immounoassay, where a BPA sample containing an anti-BPA antibody is intriduced into the SPR sendor system. The adddition of BPA into the anti-BPA antibody solution (0.8μg/ml, final concentration) was found to decrease the incidence angle shift sharply because of an inhibition effect of BPA. The RSDs (n=3) of each point were less than 4%. An evaluation of the affinity constant (K1) between anti-BPA antibody and BPA-OVA conjugate on the chip was found to be 2.26*10[6]M[-1], and that (K2) between anti-BPA antibody and free BPA (analyte) was 5.72*10[4]M[-1]. The lowest detection limit for BPA by SPR was almost the same as that by ELISA, 19[9]g/ml(1ppb).
九州大学の論文