Novel Angiotensin I-Converting Enzyme Inhibitory Peptides Found in a Thermolysin-Treated Elastin with Antihypertensive Activity
スポンサーリンク
概要
- 論文の詳細を見る
Angiotensin I-converting enzyme (ACE) inhibitory activity was generated from elastin and collagen by hydrolyzing with thermolysin. The IC50 value of 531.6 µg/mL for ACE inhibition by the elastin hydrolysate was five times less than 2885.1 µg/mL by the collagen hydrolysate. We confirmed the antihypertensive activity of the elastin hydrolysate in vivo by feeding spontaneously hypertensive rats (male) on a diet containing 1% of the elastin hydrolysate for 9 weeks. About 4 week later, the systolic blood pressure of the rats in the elastin hydrolysate group had become significantly lower than that of the control group. We identified novel ACE inhibitory peptides, VGHyp, VVPG and VYPGG, in the elastin hydrolysate by using a protein sequencer and quadrupole linear ion trap (QIT)-LC/MS/MS. VYPGG had the highest IC50 value of 244 µM against ACE and may have potential use as a functional food.
著者
-
KOBAYASHI-HATTORI Kazuo
Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture
-
Tamura Tomoko
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers
-
SHIMIZU-IBUKA Akiko
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
-
Arai Soichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
-
Sato Yuko
Department Of Applied Chemistry Faculty Of Engineering Kyushu University:jst-crest
-
Mura Kiyoshi
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
-
Nakamura Takemichi
Molecular Characterization Team Advanced Science Institute Riken
-
Toyoda Tsudoi
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
-
Mura Kiyoshi
Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture
-
SHIMIZU-IBUKA Akiko
Department of Material and Biological Chemistry, Faculty of Science, Yamagata University
-
SATO Yuko
Department of Anesthesiology, Aichi Medical University School of Medicine
関連論文
- Preparation of Hypoallergenic Wheat Flour Noodles and Evaluation of Their Physical Properties
- Left Ventricular Expression of Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 in Failing Rat Hearts
- Role of CCK-A Receptor for Pancreatic Function in Mice : A Study in CCK-A Receptor Knockout Mice
- Mechanism of Delayed Gastric Emptying in Naturally Occurring CCK-A Receptor Gene Knockout (OLETF) Rats
- Role of Cholecystokinin (CCK)-A Receptor for Pancreatic Growth After Weaning : A Study in a New Rat Model Without Gene Expression of the CCK-A Receptor
- 1P134 核様体タンパク質FisとDpsによるDNA折り畳み様式の差異(核酸-相互作用・複合体,第48回日本生物物理学会年会)
- Molecular Cloning, Characterization, and Expression of Wheat Cystatins
- Disruption of Cholecystokinin (CCK)-B Receptor Gene Did Not Modify Bile or Pancreatic Secretion of Pancreatic Growth : A Study in CCK-B Receptor Gene Knockout Mice
- Structural Organization of the Gene Encoding Corn Cystatin
- Two Distinct Species of Corn Cystatin in Corn Kernels
- Organization and Primary Sequence of Multiple Genes Encoding Type II mRNA Species of Rice Prepro-glutelin(Biological Chemistry)
- A Mainstay of Functional Food Science in Japan-History, Present Status, and Future Outlook
- Bilateral Nephrocalcinosis Associated with Distal Renal Tubular Acidosis
- Oral Administration of a Synthetic Trypsin Inhibitor Increases Pancreatic Duct Function in CCK-A Receptor-Deficient Rats
- Isolation and Characterization of a Novel Polysaccharide As a Possible Allergen Occurring in Wheat Flour
- Purification and Characterization of an α-Amylase of Pichia burtonii Isolated from the Traditional Starter "Murcha" in Nepal
- Blood Substrates and Hormonal Responses to Increased Egg White Protein Intake Prior to a 12,000m Run in Heat
- Identification of Novel Fluorescent Pyrrolopyridinium Compound Formed from Maillard Reaction of 3-Deoxyglucosone and Butylamine
- Targeted Integration of Foreign DNA Into a Defined Locus on Chromosome 19 in K562 Cells Using AAV-Derived Components
- Functional Expression and Enzymatic Properties of Two Sitophilus zeamais Cysteine Proteinases Showing Different Autolytic Processing Profiles In Vitro
- Purification and Properties of a Cysteine Proteinase Occurring in Dormant Wheat Seeds
- Characterization of a Gene Family Encoding Cysteine Proteinases of Sitophilus zeamais (Maize Weevil), and Analysis of the Protein Distribution in Various Tissues Including Alimentary Tract and Germ Cells
- Significance of the C-Terminal Domain of Erwinia uredovora Ice Nucleation-Active Protein(Ina U)
- Cloning and Sequencing of an Ice Nucleation Active Gene of Erwinia uredovora
- Large-scale Production and Purification of an Erwinia ananas Ice Nucleation Protein and Evaluation of Its Ice Nucleation Activity
- Establishment of Monoclonal Antibodies and Their Application to the Purification of Oryzacystatin(Biological Chemistry)
- Immunoassay of Oryzacystatin Occurring in Rice Seeds during Maturation and Germination(Biological Chemistry)
- 2P589 Structure and functional regulation of DNA complex formed with Cell-Signal responsive Gene Carrier(55. Drug design and delivery,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- NMR Structural Studies of a Rice Cysteine Proteinase Inhibitor, Oryzacystatin-I
- Crystallization and Preliminary X-Ray Diffraction Studies of a Rice Cysteine Proteinase Inhibitor, Oryzacystation-I
- In Vitro Assessment of a Chemically Synthesized Shiga Toxin Receptor Analog Attached to Chromosorb P (Synsorb Pk) as a Specific Absorbing Agent of Shiga Toxin 1 and 2
- Differing Effects of Water-Soluble and Fat-Soluble Extracts from Japanese Radish (Raphanus sativus) Sprouts on Carbohydrate and Lipid Metabolism in Normal and Streptozotocin-Induced Diabetic Rats
- Procyanidins are potent inhibitors of LOX-1: a new player in the French Paradox
- Primary Structure of an Allergenic Peptide Occurring in the Chymotryptic Hydrolysate of Gluten
- Fabrication and Quality Evaluation of Hypoallergenic Wheat Products
- Controlled Enzymatic Treatment of Wheat Proteins for Production of Hypoallergenic Flour
- Isolation and Cultivation of a Novel Ice Nucleation-active Strain of Xanthomonas campestris
- Freeze Concentration of Some Foodstuffs Using Ice Nucleation-active Bacterial Cells Entrapped in Calcium Alginate Gel(Food & Nutrition)
- Inhalation of nebulized nitroglycerin in dogs with experimental pulmonary hypertension induced by U46619
- Progressive Infundibular Stenosis after Balloon Pulmonary Valvuloplasty in Pulmonary Atresia with Intact Ventricular Septum in An Infant Who Underwent Brock Operation
- Sequential Interventional Cardiac Catheterization Treatment of Combined Coarctation of the Aorta and Patent Ductus Arteriosus
- Autonomic Function in Adolescents with Orthostatic Dysregulation Measured by Heart Rate Variability
- Autonomic function in Kawasaki disease with myocardial infarction : Usefulness of monitoring heart rate variability
- Intact Cell Binding for In Vitro Prediction of Sedative and Non-sedative Histamine H_1-Receptor Antagonists Based on Receptor Internalization
- Regulation of the Body Fat Percentage in Developmental-Stage Rats by Methylxanthine Derivatives in a High-Fat Diet
- Hepatitis B and C virus infection and p53 mutations in human hepatocellular carcinoma in Harbin, Heilongjian Province, China
- Construction of a Bioreactor with Immobilized Yeast Cells for Production of a Low-phenylalanine Peptide Mixture as a Phenylketonuria Foodstuff(Food & Nutrition)
- Enlarged-scale Production of a Low-phenylalanine Peptide Substance as a Foodstuff for Patients with Phenylketonuria
- Transepithelial Transport of the Bioactive Tripeptide, Val-Pro-Pro, in Human Intestinal Caco-2 Cell Monolayers(Food & Nutrition Science)
- Chemical and Nutritional Properties of Hypoallergenic Wheat Flour
- Occurrence of Bound Salt in Freeze-concentrated Soy Sauce
- Inhibitory Effect of Arphamenine A on Intestinal Dipeptide Transport
- Physicochemical Study of Calcium-binding Properties of Chemical Substances as Inhibitors against Calcium Phosphate Insolubilization
- Inhibitory Activity of Oligo- and Poly-L-glutamic Acids against Calcium Phosphate Insolubilization and Calcium Binding with Special Relevance to Their Molecular Weight Dependence
- Gene Cloning and Characterization of a Bacillus vietnamensis Metalloprotease
- Gibberellin-responsive Gene Expression Taking Place with Oryzains α and γ as Cysteine Proteinases of Rice Seeds
- Nutritional Effects of an Oligopeptide Mixture with a Very High Fischer Ratio on the Amino Acid Absorption and Cerebral Amine Metabolism in Rats Suffering from Galactosamine-induced Liver Injurry(Food & Nutrition)
- Enzymatic Modification of Zein to Produce a Non-bitter Peptide Fraction with a Very High Fischer Ratio for Patients with Hepatic Encephalopathy(Food & Nutrition)
- High-Pressure Sterilization of Ice Nucleation-active Bacterial Cells(Food & Nutrition)
- Heat-induced Gel Properties of Freeze-concentrated Egg White Produced Using Bacterial Ice Nuclei(Food & Nutrition)
- n-Alkylphenyldimethylammonium Salts as Inhibitors of the Ice Nucleating Activity of Erwinia ananas(Food & Nutrition)
- Bioavailability of L-Methionine Oligomers in Rats(Food & Nutrition)
- Interaction of an Antinucleating Chemical and an Ice Nucleation Active Bacterium : a Case Study with an n-Octylbenzyldimethylammonium Salt and Erwinia ananas(Pesticide Chemistry)
- Alkylbenzyldimethylammonium Salts as Inhibitors for the Ice Nucleating Activity of Erwinia ananas(Pesticide Chemistry)
- No Immunotoxic Effect on T Cells with Di(2-Ethylhexyl)Phthalate in Male C57BL/6 Mice
- Crystal Structure of Pyruvate Kinase from Geobacillus stearothermophilus
- Inhibition of Increases in Blood Glucose and Serum Neutral Fat by Momordica charantia Saponin Fraction
- Blockade of Salmonella enteritidis Passage across the Basolateral Barriers of Human Intestinal Epithelial Cells by Specific Antibody
- Serum Cholesterol-Decreasing Effect of Heat-Moisture-Treated High-Amylose Cornstarch in Cholesterol-Loaded Rats
- Cadmium Cation Increases the Production and mRNA Levels of Insulin-Like Growth Factor-Binding Protein-1 in HepG2
- Improvement of Digestibility, Reduction in Allergenicity, and Induction of Oral Tolerance of Wheat Gliadin by Deamidation
- Characterization and Regulation of Taurine Transport in Caco-2, Human Intestinal Cells
- Cathepsin D-Like Aspartic Proteinase Occurring in a Maize Weevil, Sitophilus zeamais, as a Candidate Digestive Enzyme
- Hypocholesterolemic Effect of Peanut Skin and Its Fractions : A Case Record of Rats Fed on a High-Cholesterol Diet
- Molecular Cloning and Characterization of an α-Amylase from Pichia burtonii 15-1
- Expression of the Stress-Related Genes for Glutathione S-Transferase and Ascorbate Peroxidase in the Most-Glycinin-Deficient Soybean Cultivar Tousan205 during Seed Maturation
- Application of an Aspergillus saitoi Protease Preparation to Soybean Curd to Modify Its Functional and Rheological Properties
- Effects of Peanut-Skin Procyanidin A1 on Degranulation of RBL-2H3 Cells
- Non-Acidic Compounds Induce the Intense Sweet Taste of Neoculin, a Taste-Modifying Protein
- Effect of Caffeine on the Body Fat and Lipid Metabolism of Rats Fed on a High-Fat Diet
- Some Properties of a Cysteine Proteinase Inhibitor from Corn Endosperm(Biological Chemistry)
- Enzymatic Synthesis of [^N]-Methionine-incorporated Peptides by Papain(Food & Nutrition)
- Oryzacystatin Exogenously Introduced into Protoplasts and Regeneration of Transgenic Rice
- Gibberellin-induced Cysteine Proteinase Occurring in Germinating Rice Seeds and Its Specificity for Digesting Oxidized Insulin B-Chain(Food & Nutrition)
- Safety Evaluation of Hypoallergenic Wheat Flour by Using a DNA Microarray
- Freezing of Water in the Presence of the Ice Nucleation Active Bacterium, Erwinia ananas, and Its Application for Efficient Freeze-drying of Foods(Food & Nutrition)
- Studies on Functional Foods in Japan : State of the Art
- Effects of Peanut-Skin Procyanidin A1 on Degranulation of RBL-2H3 Cells
- Novel Angiotensin I-Converting Enzyme Inhibitory Peptides Found in a Thermolysin-Treated Elastin with Antihypertensive Activity
- Serum Cholesterol Reduction by Feeding a High-Cholesterol Diet Containing a Lower-Molecular-Weight Polyphenol Fraction from Peanut Skin
- Novel Angiotensin I-Converting Enzyme Inhibitory Peptides Found in a Thermolysin-Treated Elastin with Antihypertensive Activity
- Peanut-Skin Polyphenols, Procyanidin A1 and Epicatechin-(4 β→6)-epicatechin-(2 β→O→7, 4 β→8)-catechin, Exert Cholesterol Micelle-Degrading Activity in Vitro
- Electrophoretical Analysis of Zein and Isolation of Its Components
- Enzymatic Modification of Protein Functionality: Implantation of Potent Amphiphilicity to Succinylated Proteins by Covalent Attachment of Leucine Alkyl Esters
- Administration of Lysinoalanine-containing Proteins to Rats and the Characterization of Their Small Intestinal Contents
- Incorporation of L-Leucine 1-13C-Dodecyl Ester to Succinylated αs1-Casein during a Papain-catalyzed Reaction
- Is Zinc Deficiency a Risk Factor of Infection in Elderly? A Pilot Study.
- Calcitonin Gene-Related Peptide Upregulates Serum Amyloid A Synthesis through Activation of Interleukin-6
- Onset Age is Associated with Outcome of Radioiodine Therapy in Graves' Disease
- Peanut-Skin Polyphenols, Procyanidin A1 and Epicatechin-(4β→6)-epicatechin-(2β→O→7, 4β→8)-catechin, Exert Cholesterol Micelle-Degrading Activity in Vitro