Peculiar Protein–Protein Interactions of the Novel Endoplasmic Reticulum Membrane Protein Rcr1 and Ubiquitin Ligase Rsp5
スポンサーリンク
概要
- 論文の詳細を見る
Overproduction of the ER membrane protein Rcr1 makes Saccharomyces cerevisiae resistant to Congo red by reducing the chitin content through a unknown mechanism. By both co-immunoprecipitation and yeast two-hybrid experiments, specific interaction between Rcr1 and the ubiquitin ligase Rsp5 was found. This binding was largely mediated by a singular VPEY sequence in Rcr1 in addition to PPSY, the consensus ligand motif of the WW domains. Mutant analysis indicated that Rsp5 and other Rcr1-interacting proteins discovered in the current screen were not engaged in Congo red resistance.
- 社団法人 日本農芸化学会の論文
著者
-
YODA Koji
Department of Biotechnology, University of Tokyo
-
Adachi Hideya
Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University
-
NODA Yoichi
Department of Biotechnology, University of Tokyo
-
ADACHI Hiroyuki
Department of Biotechnology, University of Tokyo
-
Noda Y
Univ. Tokyo Tokyo Jpn
-
Imai Keita
Department of Biotechnology, University of Tokyo
関連論文
- Analysis of splenic Gr-1^ immature myeloid cells in tumor-bearing mice
- Multidrug Resistance Phenotype Conferred by Overexpressing bfr2^+/pad1^+/sks1^+or pap1^+Genes and Mediated by bfr1^+Gene Product, a Structural and Functional Homologue of P-Glycoprotein in Schizosaccharomyces pombe
- Tryptophan Pyrolysis Products, Trp-P-1 and Try-P-2 Induce Apoptosis in Primary Cultured Rat Hepatocytes
- Cooperation of Sly1/SM-Family Protein and Sec18/NSF of Saccharomyces cerevisiae in Disassembly of cis-SNARE Membrane-Protein Complexes(Biochemistry & Molecular Biology)
- A Novel Protein, Mpml, of the Mitochondria of the Yeast Saccharomyces cerevisiae(Biochemistry & Molecular Biology)
- A Novel Membrane Protein Complex on the Endoplasmic Reticulum and Early Golgi Compartments in the Yeast Saccharomyces cerevisiae(Biochemistry & Molecular Biology)
- Hop-Resistant Lactobacillus brevis Contains a Novel Plasmid Harboring a Multidrug Resistance-Like Gene
- TmrB Protein, Which Confers Resistance to Tunicamycin on Bacillus subtilis, Binds Tunicamycin
- The Gene Responsible for Tunicamycin Resistance, tmrB, in Bacillus subtilis(Microbilolgy & Fermentation Industry)
- RENAL CARCINOGENESIS : GENOTYPE, PHENOTYPE AND DRAMATYPE
- Renal carcinogenesis : Genotype, phenotype and dramatype
- Gene Amplification of the amy E-tmrB Region in Bacillus subtilis
- Induction of Two Types of Gene Amplification by a Cloned DNA Fragment and Amplification of a Foreign Gene on the Bacillus subtilis Chromosome by Using the Fragment
- Amplification of an Integration Plasmid Introduced into the tmr A Amplification Unit of A Bacillus subtitis Tunicamycin-resistant Mutant
- Construction of New ARSI Vectors with Increased Mitotic Stability in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Mutational Site in Tunicamycin-resistant Mutation tmrB8 of Bacillus subtilis as Revealed at the Nucleotide Sequence Level(Microbiology & Fermentation Industry)
- Efficacies of Different Secretion Vectors for Secretion of Human Epidermal Growth Factor by Escherichia coli(Biological Chemistry)
- Novel Saccharomyces cerevisiae Temperature-sensitive cyrl Mutant Which Accumulates Glycogen Particles
- recE-Dependent Gene Amplification Induced by a Tunicamycin-resistant Mutation (tmrA7) in Bacillus subtilis(Microbiology & Fermentation Industry)
- Expression and Secretion of Human Epidermal Growth Factor in Escherichia coli(Microbiology & Fermentation Industry)
- Sequence of the Signal Peptide of E. coli Alkaline Phosphatase
- Distribution of Prx-linked Hydroperoxide Reductase Activity among Microorganisms
- Identification of Protein Kinase B (PKB) as a Phosphatidylinositol 3,4,5-Trisphosphate Binding Protein in Dictyostelium discoideum
- Toward chemotherapy for Tsc2-mutant renal tumor
- Peculiar Protein–Protein Interactions of the Novel Endoplasmic Reticulum Membrane Protein Rcr1 and Ubiquitin Ligase Rsp5
- In Vivo Biotinylation of Fusion Proteins Expressed in Escherichia coli with a Sequence of Propionibacterium freudenreichii Transcarboxylase 1.3S Biotin Subunit
- Vesicular Transport and the Golgi Apparatus in Yeast
- Molecular Cloning of a Gene, DHS1, Which Complements a Drug-hypersensitive Mutation of the Yeast Saccharomyces cerevisiae
- Interaction Among the Subunits of Golgi Membrane Mannosyltransferase Complexes of the Yeast Saccharomyces cerevisiae
- IMMUNOHISTOCHEMICAL ASSESSMENT OF SUPEROXIDE DISMUTASE EXPRESSION IN THE HUMAN ENDOMETRIUM THROUGHOUT THE MENSTRUAL CYCLE
- Defect in Cell Wall Integrity of the Yeast Saccharomyces cerevisiae Caused by a Mutation of the GDP-mannose Pyrophosphorylase Gene VIG9
- Molecular Characterization of a Novel Yeast Cell-wall Acid Phosphatase Cloned from Kluyveromyces marxianus
- Distinct Interaction of TmrB Protein with Membranes in Bacillus subtilis and Escherichia coli
- Electronic Spectra of Push-Pull 4-Phenylaminoazobenzene Derivatives
- Interaction of Laser Blow-Off Blob with a Low-Pressure Gas Discharge
- Mutational Analyses of Dictyostelium IQGAP-Related Protein GAPA : Possible Interaction with Small GTPases in Cytokinesis(Biochemistry & Molecular Biology)
- YW3-13 The function and role of novel protein Niban in multistep renal carcinogenesis (Young Urologist Research Workshop)
- Identification and Characterization of Two Penta-EF-Hand Ca^-Binding Proteins in Dictyostelium discoideum^1
- Bsr-REMI : An Improved Method for Gene Tagging Using a New Vector in Dictyostelium
- Inhibition of Protein Translocation in Permeabilized Cells of Schizosaccharomyces pombe by Puromycin
- Peculiar Protein-Protein Interactions of the Novel Endoplasmic Reticulum Membrane Protein Rcr1 and Ubiquitin Ligase Rsp5