Mutational Analyses of Dictyostelium IQGAP-Related Protein GAPA : Possible Interaction with Small GTPases in Cytokinesis(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
GAPA is an IQGAP-related protein and is involved in Dictyostelium cytokinesis. Since mammalian IQ-GAPs are effectors for Rac/Cdc42, GAPA is also predicted to bind to small GTPases, which are to be identified. In this study, mutant GAP As were examined for functions in cytokinesis by genetic complementation of gap A- cells. Positively charged side chains of Arg^<442> and Lys^<474> of GAPA, predicted to be present on the surface of interaction with small GTPases, were found to be essential, suggesting an interaction between GAPA and putative small GTPase in cytokinesis. Also, results from truncated GAP As indicated that almost the entire region of GAPA homologous to IQGAP is required for cytokinesis in Dictyostelium.
- 社団法人日本農芸化学会の論文
- 2001-08-23
著者
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ADACHI Hiroyuki
Department of Biotechnology, University of Tokyo
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Sutoh Kazuo
Department Of Life Sciences Graduate School Of Arts And Sciences University Of Tokyo
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Sutoh K
Department Of Life Sciences Graduate School Of Arts And Sciences University Of Tokyo
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Sakurai Masao
Department Of Life Sciences Graduate School Of Arts And Sciences University Of Tokyo
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Adachi Hiroyuki
Department Of Life Sciences Graduate School Of Arts And Sciences University Of Tokyo:(present Addres
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Adachi Hiroyuki
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo
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