Efficacies of Different Secretion Vectors for Secretion of Human Epidermal Growth Factor by Escherichia coli(Biological Chemistry)

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概要

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• We have previously constructed hEGF secretion vector pTA1522 in which phoA signal peptide-hEGF hybrid gene was expressed under the control of the phoA promoter, and have shown that Escherichia coli carrying pTA1522 produced authentic hEGF in large amounts. The present report describes the relative efficacies of a number of secretion vectors that utilized various promoters (phoA, trp, rightward and leftward promoter of phage λ), signal peptides (phoA, bta), and antibotic resistance markers (Amp^r, Km^r) in different combinations. The most effective was pTA2532 with phoA promoter, a bla signal peptide coding region, and Km^r gene. The amount of hEGF secreted was 2.12 × 10^5 molecules per cell.

• 社団法人日本農芸化学会の論文
• 1987-04-23

著者

• Yamasaki M

  Department Of Biotechnology The University Of Tokyo:(present Address)department Of Food Science And

• Yamashita Masahiro

  Department Of Bioengineering Tokyo Institute Of Technology

• YAMASAKI Makari

  Department of Agricultural Chemistry, the University of Tokyo

• YODA Koji

  Department of Biotechnology, University of Tokyo

• Yoda K

  Univ. Tokyo Tokyo Jpn

• TAMURA Gakuzo

  Department of Agricultural Chemistry, University of Tokyo

• Yamane K

  Univ. Tsukuba

• Tamura G

  Department Of Agricultural Chemistry The University Of Tokyo

• Sumi Shin-ichiro

  Central Research Laboratories Wakunaga Pharmaceutical Co. Ltd.

• Tamura Gakuzo

  Depart Ment Qf Agricultural Chemistry The University Of Tokyo

• OKA Takanori

  Central Research Laboratories, Wakunaga Pharmaceutical Co., Ltd.

• FUWA Toru

  Central Research Laboratories, Wakunaga Pharmaceutical Co., Ltd.

• MIYAKE Tetsuo

  Department of Agricultural Chemistry, The University of Tokyo

• Fuwa Toru

  Central Research Laboratories Wakunaga Pharmaceutical Co. Ltd.

• Oka Takanori

  Central Research Laboratories Wakunaga Pharmaceutical Co. Ltd.

• Miyake T

  Tosoh Co. Ltd. Ayase Jpn

• Yoda Koji

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamasaki Makari

  Department Of Agricultural Chemistry The University Of Tokyo

• Tamura Gakuzo

  Nuclear Receptor Institute Ksp

• Miyake Tetsuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Yoda K

  Institute Of Biological Sciences University Of Tsukuba

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