Evaluation of Insulin Permeability and Effects of Absorption Enhancers on Its Permeability by an in Vitro Pulmonary Epithelial System Using Xenopus Pulmonary Membrane
スポンサーリンク
概要
- 論文の詳細を見る
The permeability of insulin across Xenopus pulmonary membrane and the effects of various absorption enhancers on insulin permeability were examined using an in vitro Ussing chamber technique. Absorption enhancers used in this study were sodium caprate(NaCap), sodium glycocholate(NaGC), sodium salicylate(NaSal) and ethylenediaminetetraacetic acid disodium salt(EDTA). The permeability of insulin across Xenopus pulmonary membrane significantly increased in the presence of NaCap and NaGC, while EDTA and NaSal did not enhance the permeability. In addition, the enhancing effect of NaGC increased as the concentrations of these enhancers increased. Transmembrane resistance(Rm) of Xenopus lung was markedly decreased in the presence of these enhancers, and NaCap showed a greater effect on Rm than NaGC. Furthermore, the amount of alkaline phosphatase(ALP) and lactate dehydrogenase(LDH) released from the apical side of the Xenopus pulmonary membrane increased in the presence of these enhancers. These results indicate that NaCap and NaGC improve the pulmonary absorption of insulin, but they are toxic to the pulmonary membrane. These findings suggest that this method is useful for estimating the permeability characteristics of peptides across the pulmonary membrane and for evaluating the effects of various additives on their permeability and their membrane toxicity.
- 社団法人日本薬学会の論文
- 2001-04-01
著者
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Okada Naoki
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
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Yamashita Masahiro
Department Of Bioengineering Tokyo Institute Of Technology
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YAMAMOTO Akira
Department of Biopharmaceutics, Kyoto Pharmaceutical University
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MURANISHI Shozo
Department of Biopharmaceutics, Kyoto Pharmaceutical University
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FUJITA Takuya
Department of Biopharmaceutics, Kyoto Pharmaceutical University
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TANAKA Hideaki
Department of Gastroenterology and Hepatology, Division of Clinical Medicine and Biomedical Science,
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Fujita T
Division Of Oral Cytology And Cell Biology Department Of Developmental And Reconstructive Medicine N
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Okada N
Graduate School Of Pharmaceutical Sciences Osaka University
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FUJITA Takuya
Ritsumeikan University College of Information Science and Engeneering
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OKUMURA Shigeki
Department of Biopharmaceutics, Kyoto Pharmaceutical University
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SHINSAKO Keiko
Department of Biopharmaceutics, Kyoto Pharmaceutical University
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ITO Mayuko
Department of Biopharmaceutics, Kyoto Pharmaceutical University
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Ito Mayuko
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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Shinsako Keiko
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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Okumura Shigeki
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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Yamamoto Akira
Dep. Of Biopharmaceutics Kyoto Pharmaceutical Univ.
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Fujita T
Ritsumeikan University College Of Information Science And Engeneering
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Fujita Takuya
Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University
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Okada Naoki
Department Of Bioppharmaceutics Kyoto Pharmaceutical University
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Fujita T
Showa College Of Pharmaceutical Sciences
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Fukushima Tohru
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Setsunan University
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Tanaka Hideaki
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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村西 昌三
京都薬大
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Muranishi Shozo
Department Of Biopharmaceutics Kyoto Pharmaceutical University:exploratory Development Laboratories
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Muranishi Shozo
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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Yamamoto Akira
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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Okada Naoki
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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Yamamoto Akira
Department Of Anatomy School Of Medicine University Of Tokushuma
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Yamashita Masahiro
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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Tanaka Hideaki
Department Of Applied Chemistry & Biochemistry Faculty Of Engineering Kumamoto University
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Yamamoto Akira
Department Of Anatomy School Of Medicine University Of Tokushima
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