Cloning Genomic DNA Encoding Apple Polyphenol Oxidase and Comparison of the Gene Product in Escherichia coli and in Apple
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概要
- 論文の詳細を見る
Two PCR-amplified genomic DNA fragments encoding apple(cv.Fuji)polyphenol oxidase(PPO)were cloned and sequenced. A comparison of genomic DNA with cDNAs revealed that the PPOs lacked introns. Both PPO DNAs appear to encode a 66-kDa precursor protein consisting of a 56-kDa mature protein and a N-terminal transit peptide of 10-kDa N-terminal transit peptide. Apple PPO DNA was expressed in Escherichia coli, and the gene product(56kDa)without a transit peptide was immunochemically detected and was the same size(ca.65kDa)as the main PPO of apple fruit by SDS-PAGE.
- 社団法人日本農芸化学会の論文
- 1998-02-23
著者
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Yamasaki M
Department Of Biotechnology The University Of Tokyo:(present Address)department Of Food Science And
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YAMASAKI Makari
Department of Agricultural Chemistry, the University of Tokyo
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Murata Michiyo
Kacho Jr. College
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Murata M
Nara University Of Education
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Murata Michiyo
Nara University Of Education
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KADOKURA HIROSHI
Department of Biotechnology, The University of Tokyo
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MURATA Masatsune
Department of Nutrition and Food Science, Ochanomizu University
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Murata Masakazu
Marine Biochemistry Division National Research Institute Of Fisheries Science
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HOMMA Seiichi
Department of Nutrition and Food Science, Ochanomizu University
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Shimizu Seki
Department of Biology, Faculty of Science, Ochanomizu University
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Yamasaki Makari
Department Of Agricultural Chemistry The University Of Tokyo
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Yamasaki Makari
Department Of Biotechnology University Of Tokyo
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SAKUTA Masaaki
Department of Biology, Ochanomizu University
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Kadokura Hiroshi
Department Of Biotechnology The University Of Tokyo
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Kadokura Hiroshi
Department Of Biotechnology University Of Tokyo
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Sakuta M
Department Of Biology Ochanomizu University
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Shimizu Seki
Department Of Biology Faculty Of Science Ochanomizu University
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HARUTA Miyoshi
Department of Nutrition and Food Science, Ochanomizu University
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HIRAIDE Ayami
Department of Nutrition and Food Science
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Homma S
Department Of Nutrition And Food Science Ochanomizu University
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Homma Seiichi
Department Of Nutrition & Food Science Ochanomizu University
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Haruta Miyoshi
Department Of Nutrition And Food Science Ochanomizu University
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Sakuta Masaaki
Department Of Biological Sciences Ochanomizu University
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Murata Masatsune
Department Of Nutrition & Food Science Ochanomizu University
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HOMMA Seiichi
Department of Bioscience, Tokyo University of Agriculture
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