Subcellular Location of Polyphenol Oxidase in Apples
スポンサーリンク
概要
- 論文の詳細を見る
The location of polyphenol oxidase (PPO) in cells of apple fruit was examined by immunohistochemistry and subcellular fractionation. In mature apple fruits, where vacuoles occupy most of the cells, PPO was detected immunochemically near the cell walls with use of anti-apple PPO antibodies. In cells of immature fruits and tissue culture, PPO was detected in organelles other than the vacuoles, probably in plastids. The plastid fraction was purified by density gradient ultracentrifugation, and the activities of PPO and marker enzymes of plastids were the highest in the plastids. Most apple PPO was in plastids, as are other plant PPOS, and Some of the protein was solubilized and proteolyzed during ripening and storage.
- 社団法人日本農芸化学会の論文
- 1997-09-23
著者
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Murata Michiyo
Kacho Jr. College
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Murata M
Nara University Of Education
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Murata Michiyo
Nara University Of Education
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MURATA Masatsune
Department of Nutrition and Food Science, Ochanomizu University
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Murata Masakazu
Marine Biochemistry Division National Research Institute Of Fisheries Science
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HOMMA Seiichi
Department of Nutrition and Food Science, Ochanomizu University
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TSURUTANI Mie
Department of Nutrition and Food Science, Ochanomizu University
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HAGIWARA Sonoko
Department of Nutrition and Food Science, Ochanomizu University
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Tsurutani Mie
Department Of Nutrition And Food Science Ochanomizu University
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Homma S
Department Of Nutrition And Food Science Ochanomizu University
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Homma Seiichi
Department Of Nutrition & Food Science Ochanomizu University
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Hagiwara Sonoko
Department Of Nutrition And Food Science Ochanomizu University
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Murata Masatsune
Department Of Nutrition & Food Science Ochanomizu University
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HOMMA Seiichi
Department of Bioscience, Tokyo University of Agriculture
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