Comparison of Soluble and Plastidal Polyphenol Oxidase in Mature Apples
スポンサーリンク
概要
- 論文の詳細を見る
Polyphenol oxidase (PPO) is transported to plastid after translation. However, we often detect PPO activity in a soluble fraction of apple. Here we examined the location of PPO in retail apples. PPO activity was detected primarily in the plastidal fraction in most apples, however, it was sometimes detected more strongly in the soluble fraction than in the plastidal fraction. Western blotting analysis detected PPOs in both plastidal and soluble fractions as the same band. Some apples having high soluble PPO activity showed a band of decomposed PPO in addition to an intact band. Plastidal, soluble, and solubilized PPOs had similar optimal pH at about 4 and Km values (about 100 μM). However, solubilized PPO showed the highest Vmax and soluble PPO the least value. Some part of PPO seems to be solubilized, denatured and proteolyzed during maturation and storage.
- 社団法人 日本食品科学工学会の論文
- 2002-02-01
著者
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Murata Michiyo
Kacho Jr. College
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Murata M
Nara University Of Education
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Murata Michiyo
Nara University Of Education
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MURATA Masatsune
Department of Nutrition and Food Science, Ochanomizu University
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Murata Masakazu
Marine Biochemistry Division National Research Institute Of Fisheries Science
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HOMMA Seiichi
Department of Nutrition and Food Science, Ochanomizu University
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TSURUTANI Mie
Department of Nutrition and Food Science, Ochanomizu University
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YANAGIDA Yasuko
Department of Nutrition and Food Science, Ochanomizu University
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HAGIWARA Sonoko
Department of Nutrition and Food Science, Ochanomizu University
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Tsurutani Mie
Department Of Nutrition And Food Science Ochanomizu University
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Homma S
Department Of Nutrition And Food Science Ochanomizu University
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Homma Seiichi
Department Of Nutrition & Food Science Ochanomizu University
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Yanagida Yasuko
Department Of Nutrition And Food Science Ochanomizu University
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Hagiwara Sonoko
Department Of Nutrition And Food Science Ochanomizu University
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Murata Masatsune
Department Of Nutrition & Food Science Ochanomizu University
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HOMMA Seiichi
Department of Bioscience, Tokyo University of Agriculture
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Yanagida Yasuko
Department of Mechano-Micro Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Yokohama 226-8502, Japan
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YANAGIDA Yasuko
Department of Mechano-Micro Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology
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