Purification of Stearidonic Acid (18 : 4 (n-3)) and Hexadecatetraenoic Acid (16 : 4 (n-3)) from Algal Fatty Acid with Lipase and Medium Pressure Liquid Chromatography
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概要
- 論文の詳細を見る
Stearidonic acid (18 : 4 (n-3)) and hexadecatetraenoic acid (16 : 4 (n-3)) are included in some edible marine algae such as Undaria pinnatifida and Uiva pertusa with relatively high compositions (up to 40%) of total fatty acids. In order to prepare 16 : 4 (n-3) and 18 : 4 (n-3) enriched fatty acid concentrates, we screened for a suitable lipase which concentrates these acids by the removal of other fatty acids in the selective esterification reaction reported by Shimada et al. (Shimada et al. (1997), J. Am. Oil Chem. Soc., 74,1465-1470). In combination with the lipase reaction and reversed-phase medium pressure liquid chromatography, we purified 18 : 4 (n-3) and 16 : 4 (n-3) to more than 95% purity.
- 社団法人日本農芸化学会の論文
- 2000-11-23
著者
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Murata Michiyo
Kacho Jr. College
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KOMATSU Wataru
Department of Applied Biological Science, Tokyo Noko University
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Murata M
Nara University Of Education
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Murata Michiyo
Nara University Of Education
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Komatsu Wataru
Department Of Life Science Toita Women's College
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Murata Masakazu
Marine Biochemistry Division National Research Institute Of Fisheries Science
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SHINOHARA Kazuki
National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, Tsukuba
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SAITO HIROAKI
Tohoku National Fisheries Research Institute
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Saito H
Marine Biochemistry Division National Research Institute Of Fisheries Science
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ISHIHARA Kenji
Marine Biochemistry Division, National Research Institute of Fisheries Science
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KANENIWA Masaki
Marine Biochemistry Division, National Research Institute of Fisheries Science
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SAITO Hiroaki
Marine Biochemistry Division, National Research Institute of Fisheries Science
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SAITO Hiroaki
Tokai Regional Fisheries Research Laboratory, Fisheries Agency
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Ishihara K
Department Of Food And Nutrition School Of Life Studies Sugiyama Jogakuen University
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Kaneniwa M
Marine Biochemistry Division National Research Institute Of Fisheries Science
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Kaneniwa Masaki
Marine Biochemistry Division National Research Institute Of Fisheries Science
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Shinohara K
National Food Research Institute
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Shinohara Kazuki
National Food Res. Inst. Ibaraki Jpn
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Shinohara Kazuki
Laboratory Of Food Chemistry Faculty Of Agriculture Kyushu University
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Saito H
National Research Institute Of Fisheries Science Fisheries Research Agency
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Saito Hiroaki
Laboratory Of Lipid Chemistry National Research Institute Of Fisheries Science Ministry Of Agricultu
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Ishihara Kenji
Marine Biochemistry Division National Research Institute Of Fisheries Science
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Saito Hiroaki
Fisheries Oceanography Tohoku National Fisheries Research Institute
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Saito Hiroaki
Marine Biochemistry Division National Research Institute Of Fisheries Science
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Saito Hiroaki
Laboratory Of Lipid Chemistry National Research Institute Of Fisheries Science Ministry Of Agricultu
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Shinohara Kazuki
Food Chemistry Institute Faculty Of Agriculture Kyushu University
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