Competitive ELISA of Bovine Lactoferrin with Bispecific Monoclonal Antibodies
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概要
- 論文の詳細を見る
A mouse hybrid-hybridoma, HH1-4-3, secreting IgG_1 class bispecific antibodies to bovine lactoferrin (bLF) and horseradish peroxidase (HRPO), was established previously. The competitive enzyme-linked immunosorbent assay (ELISA) of bLF using the HH1-4-3 culture supernatant was not sensitive enough to measure bLF concentration in biological fluids. To improve the sensitivity of the competitive ELISA, we fractionated the bispecific antibodies by antigen affinity column chromatography. A column immobilized with bLF adsorbed 60%o of the antibodies of the HH1-4-3 supernatant, and the amount of antibodies adsorbed on a column immobilized with HRPO was less than 5%. The competitive ELISA of bLF using the affinity purified bispecitio antibodies through an HRPO-immobilized column chromatography showed a good standard curve at bLF concentrations of 10 ng/ml to 100 μg/ml.
- 社団法人日本農芸化学会の論文
- 1997-06-23
著者
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Takahashi T
Department Of Sustainable Resource Science Faculty Of Bioresources Mie University
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SHINOHARA Kazuki
National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, Tsukuba
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SHINMOTO Hiroshi
National Food Research Institute
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Hirose S
Tokachi‐ikeda Research Inst. Viticulture And Enology Hokkaido Jpn
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Tsushida T
National Food Res. Inst. Tsukuba Jpn
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Tsushida Tojiro
National Food Research Institute
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Tsushida Tojiro
National Research Institute Of Vegetables Ornamental Plants And Tea Department Of Tea Processing Tec
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Shinmoto H
Snow Brand Milk Products Co. Ltd. Kawagoe Jpn
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Kobori M
National Food Research Institute
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KUBORI Masuko
National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries
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Tsushida Tojiro
National Food Res. Inst. Tsukuba Jpn
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Shinohara K
National Food Research Institute
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Shinohara Kazuki
National Food Res. Inst. Ibaraki Jpn
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Kobori Masuko
National Food Research Institute
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Shinohara Kazuki
Laboratory Of Food Chemistry Faculty Of Agriculture Kyushu University
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Shinohara Kazuki
Food Chemistry Institute Faculty Of Agriculture Kyushu University
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