Purification and Characterization of Tora-mame (Phaseolus vulgaris) Seed Calmodulin(Biological Chemistry)
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概要
- 論文の詳細を見る
We purified calmodulin to apparent homogeneity from Tora-mame (Phaseolus vulgaris) seeds, one of the Japanese cultivars of the common bean. Electrophoretically, the purified Tora-mame calmodulin migrated faster than bovine brain calmodulin in the presence or absence of Ca^<2+>. The estimated molecular weight of Tora-mame calmodulin was 14,200 in the presenc of Ca^<2+> and 18,200 in the absence of Ca^<2+>. Like other plant calmodulin, Tora-mame calmodulin lacked tryptophan and contained 1 mol each of tyrosine and half cystine per mol of protein. However, Tora-mame calmodulin has fewer acidic amino acid residues than other plant calmodulins did. Rat brain phosphodiesterase (Ca^<2+>-PDE) was stimulated by Tora-mame calmodulin, but the concentration of Tora-mame calmodulin, giving the half-maximal activation of Ca^<2+>-PDE, was higher than that of bovine calmodulin, and the maximal activity of Ca^<2+>-PDE obtained with Tora-mame calmodulin was lower than that obtained with bovine protein. The Ki value of W-7, a calmodulin antagonist, for Tora-mame calmodulin (17μM) was larger than that for bovine calmodulin (8μM). These observations suggest that hydrophobic regions of Tora-mame calmodulin exposed by Ca^<2+>-induced conformational changes were slightly different from those of bovine calmodulin.
- 社団法人日本農芸化学会の論文
- 1990-10-23
著者
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TAKAHASHI Takao
Department of Surgical Oncology, Gifu University
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Tanaka Toshio
Department Of Respiratory Medicine Allergy And Rheumatic Disease Osaka University Graduate School Of
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Takahashi T
Department Of Sustainable Resource Science Faculty Of Bioresources Mie University
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Tsushida T
National Food Res. Inst. Tsukuba Jpn
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Tsushida Tojiro
National Research Institute Of Vegetables Ornamental Plants And Tea Department Of Tea Processing Tec
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Umekawa Hayato
Department Of Sustainable Resource Science Faculty Of Bioresources Mie University
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FURUICHI Yukio
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University
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Furuichi Y
Faculty Of Bioresouces Mie University
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Umekawa H
Mie Univ. Tsu Jpn
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Umekawa Hayato
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University
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TAMIYA Toshinaga
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University
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Takahashi Takao
Department Of Breast Oncology Saitama International Medical Center Saitama Medical University
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Tanaka Toshio
Department Of Cardiology Matsusaka Central General Hospital
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Tamiya Toshinaga
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University
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Tanaka Toshio
Department Of Applied Physics Faculty Of Engineering Tokyo University
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FURUICHI Yukio
Department of Agricultural Chemistry, Faculty of Agriculture, Mie University
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Tanaka Toshio
Department of Applied Chemistry, Faculty of Engineering, Osaka Univeristy
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