Characterization of a Lectin from the Leaves of Great Northern Bean, Phaseolus vulgaris L.

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概要

• 論文の詳細を見る

• A novel lectin (GN_LL) was isolated from the leaves of the Great Northern bean, Phaseolus vulgaris. GN_LL was purified by affinity chromatography on ovomucoid-Sepharose 4B. GN_LL had a molecular mass of 135 kDa on gel filtration and gave two bands on SDS-polyacrylamide gel electrophoresis (PAGE) (band A of 34.0 kDa and band B of 34.2 kDa). Binding assay of horseradish peroxidase (HRP)-glycoproteins to the bands electroblotted onto polyvinylidene difluoride (PVDF) membrane showed that both bands could bind to complex-type N-linked oligosaccharide chains in glycoproteins. The N-terminal amino acid sequences of both bands were identical through the 10 residues and identical to that of α-subunit of a pod lectin (pod-α-subunit) from the same bean. On the other hand, band B cross-reacted with monoclonal antibody against a seed lectin from the same bean, but band A did not.

• 社団法人日本農芸化学会の論文
• 1996-04-23

著者

• TAKAHASHI Takao

  Department of Surgical Oncology, Gifu University

• Takahashi T

  Department Of Sustainable Resource Science Faculty Of Bioresources Mie University

• OZEKI Munetaka

  Department of Forensic Medicine and Molecular Pathology, Graduate School of Medicine, Kyoto Universi

• Tsushida T

  National Food Res. Inst. Tsukuba Jpn

• Tsushida Tojiro

  National Research Institute Of Vegetables Ornamental Plants And Tea Department Of Tea Processing Tec

• Umekawa Hayato

  Department Of Sustainable Resource Science Faculty Of Bioresources Mie University

• KAMEMURA Kazuo

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University

• FURUICHI Yukio

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University

• Kamemura Kazuo

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University

• Furuichi Y

  Faculty Of Bioresouces Mie University

• Umekawa H

  Mie Univ. Tsu Jpn

• Umekawa Hayato

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University

• Ozeki Munetaka

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University

• Takahashi Takao

  Department Of Breast Oncology Saitama International Medical Center Saitama Medical University

• FURUICHI Yukio

  Department of Agricultural Chemistry, Faculty of Agriculture, Mie University

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