Purification and Characterization of Three Mitogenic Lectins from the Roots of Pokeweed : (Phytolacca americana)
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概要
- 論文の詳細を見る
Three mitogenic lectins, designated PL-A, PL-B, and PL-C, were purified from the roots of pokeweed (Phytolacca americana) using Q-Sepharose column chromatography followed by gel filtration on Sephadex G-75, hydrophobic chromatography on Butyl-Toyopearl, and FPLC on a Mono-Q column. PL-A, PL-B, and PL-C are acidic proteins having isoelectric points of 4. 35 and their apparent molecular masses were 22, 48, and 21kDa by SDS-polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol, respectively. The three lectins have similar amino acid compositions rich in half-cystine and similar N-terminal sequences, indicating that they are homologous proteins. Identical sequences of N-terminal regions and six corresponding tryptic peptides in PL-A and PL-B suggested that PL-A may be an N-terminal half fragment of PL-B. Although all of three lectins have mitogenic activities, PL-B is a mitogenic lectin with the most potent hemagglutinating and mitogenic activities, and PL-C has almost no hemagglutinating activity.
- 社団法人日本農芸化学会の論文
- 1995-04-23
著者
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FUNATSU Gunki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Yamaguchi K
Kyushu Univ. Fukuoka Jpn
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Yamaguchi K
Sagami Chemical Res. Center Kanagawa Jpn
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Yamaguchi Ken-ichi
Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University
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Umekawa Hayato
Department Of Sustainable Resource Science Faculty Of Bioresources Mie University
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Umekawa Hayato
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University
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Funatsu G
Kyushu Univ. Fukuoka Jpn
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Funatsu Gunki
Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric
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Kino Mitsutaka
Laboratory of Protein Chemistry and Engineering, Faculty of Agriculture, Kyushu University
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Yamaguchi Kohji
Tonen Corporation
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Kino M
Kyowa Hakko Kogyo Co. Ltd. Yamaguchi Jpn
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