Isolation and Amino Acid Sequences of Two Trypsin Inhibitors from the Seeds of Bitter Gourd (Momordica charantia)

スポンサーリンク

概要

• 論文の詳細を見る

• Two novel trypsin inhibitors, MCTI-II' and BGIT, were isolated from the seeds of bitter gourd (Momordica charantia) and their amino acids sequenced. MCTI-II' was a squash type trypsin inhibitor and lacked the N-terminal arginine residue of Momordica charantia trypsin inhibitor (MCTI)-II. It consists of 27 amino acid residues and forms a dimeric structure. BGIT had 88% homology with bitter gourd inhibitor against an acidic amino acid-specific endopeptidase (BGIA) consisting of 68 amino acid residues and inhibited not only trypsin but also subtilisin Carlsberg. The amino acid replacements occurred in 8 positions of which that of Gln2 by Arg or of Ala44 by Lys is suggested to be responsible for the trypsin-inhibitory action of BGIT. Inhibitory activity of BGIT for trypsin was greatly decreased by acetylation, while that for subtilisin was slightly increased. From these results and the sequence comparison with eglin-c superfamily inhibitos, the reactive site of BGIT is assumed to be Lys44

• 社団法人日本農芸化学会の論文
• 1995-03-23

著者

• Miura Susumu

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

関連論文

• Some Properties of Ricin D Modified with a Methoxypolyethylene Glycol Derivative(Biological Chemistry)
• Further Studies on the Histidine Residue Located at the High Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
• Identification of the Tryptophan Residue Present in the Low Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
• Significance of the Histidine Residue at the High Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
• Significance of the Tryptophan Residue at the Low Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
• Chemical Modification of Ricin D with N-Acetylimidazole(Biological Chemistry)
• The Interaction of Castor Bean Lectins with Galactomannan(Biological Chemistry)
• Denaturation of Ricin D in Frozen Aqueous Solutions(Biological Chemistry)
• Base Specificity of Two Plant Seed Ribonucleases from Momoridica charantia and Luffa cylindrica
• Brefeldin A Protects Ricin-Induced Cytotoxicity in Human Cancer KB Cell Line, but not in its Resistant Counterpart with Altered Golgi Structures
• Primary Structure of 6.5k-Arginine/Glutamate-rich Polypeptide from the Seeds of Sponge Gourd (Luffa cylindrica)
• Chemical Modifications of Momordin-a and Luffin-a, Ribosome-Inactivating Proteins from the Seeds of Momordica charantia and Luffa cylindrica : Involvement of His140,Tyr165,and Lys231 in the Protein-Synthesis Inhibitory Activity
• Amino Acid Sequences of the Two Smallest Trypsin Inhibitors from Sponge Gourd Seeds(Biological Chemistry)
• Isolation and Amino Acid Sequence of a Protein-synthesis Inhibitor from the Seeds of Rye (Secale cereale)
• Purification and Characterization of Three Mitogenic Lectins from the Roots of Pokeweed : (Phytolacca americana)
• Complete Amino Acid Sequence of Luffin-a, a Ribosome-inactivating Protein from the Seeds of Luff a cylindrica(Biological Chemistry)
• Complete Amino Acid Sequence of Luffin-b, a Ribosome-Inactivating Protein from Sponge Gourd (Luffa cylindrica) Seeds(Biological Chemistry)
• Structural Analysis of N-Linked Oligosaccharide of Mitogenic Lectin-B from the Roots of Pokeweed (Phytolacca americana)
• Purification of Chymotrypsin Inhibitors from Larval Hemolymph of the Silkworm, Bombyx mori
• Expression and Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity in a Ribonuclease MC1 from the Seeds of Bitter Gourd
• The Amino Acid Sequence of Mitogenic Lectin-B from the Roots of Pokeweed : Phytolacca americana
• Amino Acid Sequence and Some Properties of Lectin-D from the Roots of Pokeweed (Phytolacca americana)
• The Complete Amino Acid Sequence of Lectin-C from the Roots of Pokeweed (Phytolacca americana)
• Role of the Histidine Residue at the Saccharide-binding Site of Castor Bean Hemagglutinin(Biological Chemistry)
• Chemical Modification of Castor Bean Hemagglutinin with Diethylpyrocarbonate(Biological Chemistry)
• States and Roles of Tryptophan Residues in Abrus precatorius Agglutinin(Biological Chemistry)
• Identification of the Tryptophan Residue Located at the Saccharide Binding Site of Castor Bean Hemagglutinin(Biological Chemistry)
• Purification and Characterization of Abrus Precatorius agglutinin
• Binding of Saccharides to Abrin-b Isolated from Abrus precatorius Seeds
• Chemical Modification of Tryptophan Residues in Abrin-a(Biological Chemistry)
• The Interaction of Abrin-a with Saccharides as Analyzed by Equilibrium Dialysis and Spectroscopy(Biological Chemistry)
• Spectroscopic Analysis of the Interaction of Ricin D with Urea(Biological Chemistry)
• Hydrophobicities of Ricin D and Its Constituent Polypeptide Chains(Biological Chemistry)
• Complete Amino Acid Sequence of Luffin-a, a Ribosome-inactivating Protein from the Seeds of Sponge Gourd (Luffa cylindrica)(Biological Chemistry)
• Isolation and Characterization of Two Momordins, Ribosome-Inactivating Proteins from the Seeds of Bitter Gourd (Momordica charantia)
• Formation of Oligosaccharides from Lactose by Bacillus circulans β-Galactosidase
• Positions of Disulfide Bonds in Rye (Secale cereale) Seed Chitinase-a
• Complete Amino Acid Sequence of Chitinase-A from Leaves of Pokeweed (Phytolacca americana)
• The Complete Amino Acid Sequence of Chitinase-B from the Leaves of Pokeweed (Phytolacca americana)
• Primary Structures of N-Linked Oligosaccharides of Momordin-a, a Ribosome-inactivating Protein from Momordica charantia Seeds(Biological Chemistry)
• Purification and Characterization of Two Chitinases from the Leaves of Pokeweed (Phytolacca americana)
• The Complete Amino Acid Sequences of the B-Chains of Abrin-a and Abrin-b, Toxic Proteins from the Seeds of Abrus precatorius
• Isolation of Glycopeptides from Ricinus communis Lectins(Biological Chemistry)
• Isolation and Characterization of Two Luffins, Protein-biosynthesis Inhibitory Proteins from the Seeds of Luffa cylindrica(Biological Chemistry)
• Involvement of C-Terminal Region in Inhibition of Protein Synthesis by Ricin A-Chain(Biological Chemistry)
• Purification and Characterization of the Constituent Polypeptide Chains of Abrus precatorius Agglutinin(Biological Chemistry)
• N-Acetyl-D-glucosamine-Asparagine Structure in Ribosome-inactivating Proteins from the Seeds of Luffa cylindrica and Phytolacca americana(Biological Chemistry)
• Isolation and Partial Characterization of Three Protein-synthesis Inhibitory Proteins from the Seeds of Luffa cylindrica(Biological Chemistry)
• The Amino Acid Sequence of Barley Rootlet Trypsin Inhibitor(Biological Chemistry)
• Significance of Carboxyl Group Present in the High Affinity Saccharide-binding Site of Ricin D(Biological Chemistry)
• Involvements of Trp23 in the Chitin-binding and of Trp131 in the Chitinase Activity of Rye Seed Chitinase-a
• Identification of the Tryptophan Residue Located at the Substrate-binding Site of Rye Seed Chitinase-c
• Effects of Chemical Modification of Arginine Residues Outside the Active Site Cleft of Ricin A-Chain on Its RNA N-Glycosidase Activity for Ribosomes
• Structural Analyses of Sugar Chains from Ricin A-Chain Variant(Biological Chemistry)
• Structures of Novel Acidic Galactooligosaccharides Synthesized by Bacillus circulans β-Galactosidase
• Isolation and Amino Acid Sequences of Two Trypsin Inhibitors from the Seeds of Bitter Gourd (Momordica charantia)
• Isolation and Molecular Characterization of Four Arginine/Glutamate Rich Polypeptides from the Seeds of Sponge Gourd (Luffa cylindrica)
• Actions of Pokeweed Antiviral Protein on Virus-infected Protoplasts
• The Complete Amino Acid Sequence of the A-Chain of Abrin-a, a Toxic Protein from the Seeds of Abrus precatorius(Biological Chemistry)
• Isolation from α-Zein of Thermolysin Peptides with Angiotensin I-Converting Enzyme Inhibitory Activity
• Limited Proteolysis and Reduction-carboxymethylation of Rye Seed Chitinase-a: Role of the Chitin-binding Domain in Its Chitinase Action
• Effects of Salt Concentration on Translational Ability of Ricin A-Chain-treated Ribosomes(Biological Chemistry)
• The Complete Amino Acid Sequence of Momordin-a, a Ribosome-inactivating Protein from the Seeds of Bitter Gourd (Momordica charantia)
• Purification and Some Properties of a β-Xylosidase and an α-Fucosidase from Apple Snails (Pomacea canaliculata)
• The Complete Amino Acid Sequence of Chitinase-a from the Seeds of Rye (Secale cereal)
• The Complete Amino Acid Sequence of Chitinase-c from the Seeds of Rye (Secale cereal)
• Involvement of the B Chain C-Terminal Region in the High-affinity Saccharide Binding Site of Ricin D(Biological Chemistry)
• Identification of the Aspartic Acid Residue Located at or near Substrate-binding Site of Rye Seed Chitinase-c
• Purification and Some Properties of Three Chitinases from the Seeds of Rye (Secale cereale)
• The Complete Amino Acid Sequence of Antiviral Protein from the Seeds of Pokeweed (Phytolacca americana)(Biological Chemistry)
• Binding of Lactose and Galactose to Native and lodinated Ricin D
• Identification of Tyrosyl Residue Present in the High-affinity Saccharide-binding Site of Ricin D
• Induction of Superoxide Production in Polymorphonuclear Leucocytes by Ricinus Lectins and Its Dependency on Lectin Valency
• Chemical Modification of Tryptophan Residues in Castor Bean Hemagglutinin

もっと見る 閉じる

スポンサーリンク