Chemical Modifications of Momordin-a and Luffin-a, Ribosome-Inactivating Proteins from the Seeds of Momordica charantia and Luffa cylindrica : Involvement of His140,Tyr165,and Lys231 in the Protein-Synthesis Inhibitory Activity

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概要

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• Effects of chemical modifications on the protein-synthesis inhibitory (PSI) activities of momordin-a and luffin-a were investigated. Treatment with a 50-fold excess of diethylpyrocarbonate at pH 6.5 modified one histidine residue in momordin-a and luffin-a and reduced their PSI activities to 10% and 8.3%, respectively. Modifications with a 20-fold excess of KI_3 at pH 7.0 at 0℃ greatly reduced their PSI activities to 10% by iodination of nearly one tyrosine residue. The PSI activity of momordin-a was rapidly reduced to 6.4% by the modification of one lysine residue with trinitrobenzensulfonic acid as in the case of luffin-a reported previously. By analyses of the tryptic peptides from the modified momordin-a and luffin-a, the modified residues were identified as His140,Tyr165,and Lys231. Furthermore, the amounts of three modified momordin-a binding to rat liver ribosomes were reduced to about half or less than half of that of native momordin-a. From these results, it was suggested that His140,Tyr165,and Lys231 are highly exposed on the surface of momord-in-a and luffin-a molecules and are involved in their PSI activities, probably by binding to ribosomes.

• 社団法人日本農芸化学会の論文
• 1998-05-23

著者

• FUNATSU Gunki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Islam M

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• MINAMI Yuji

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• ISLAM M.Rafiqul

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Minami Yuji

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University:(present Address)department Of B

• Funatsu Gunki

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Minami Yuji

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

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