The Complete Amino Acid Sequence of Chitinase-B from the Leaves of Pokeweed (Phytolacca americana)

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概要

• 論文の詳細を見る

• The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 tryptic peptides derived from the reduced and S-carboxymethylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecular mass of 29,473 Da. Six cysteine residues are linked by disulfide bonds between Cys20 and Cys67, Cys50 and Cys57, and Cys159 and Cys188. From 58-68% sequence homology of PLC-B with five class III chitinases, it was concluded that PLC-B is a basic class III chitinase.

• 社団法人日本農芸化学会の論文
• 1995-05-23

著者

• Yanagita T

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• FUNATSU Gunki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Yanagita Teruyoshi

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• Funatsu G

  Kyushu Univ. Fukuoka Jpn

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• YAMAGAMI Takeshi

  Laboratories of Protein Chemistry and Engineering

• Yamagami T

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci

• TANIGAWA Miho

  Laboratory of Protein Chemistry & Engineering, Department of Genetic Resources Technology, Graduate

• Tanigawa Miho

  Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Graduat

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