Antifungal Activity of Rye (Secale cereale) Seed Chitinases : the Different Binding Manner of Class I and Class II Chitinases to the Fungal Cell Walls(Biochemistry & Molecular Biology)

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概要

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• The antifungal activities of rye seed chitinase-a (RSC-a, class I) and -c (RSC-c, class II) were studied in detail using two different bioassays with Trichoderma sp. as well as binding and degradation experiments with the cell walls prepared from its mycelia. RSC-a inhibited more strongly the re-extension of the hyphae, containing mainly mature cells, than RSC-c did. Upon incubation of the fungus with fluorescent chitinases, FITC-labeled RSC-a was found to be located in the hyphal tips, lateral walls, and septa, while FITC-labeled RSC-c was only in the hyphal tip. RSC-a had a greater affinity for the cell walls than RSC-c. RSC-a liberated a larger amount of reducing sugar from the cell walls than RSC-c did. These results inferred that RSC-a first binds to the lateral walls and septa, consisting of the mature cell walls, and degrades mature chitin fiber, while RSC-c binds only to the hyphal tip followed by degradation of only nascent chitin. As a result, RSC-a inhibited fungal growth more effectively than RSC-c. Furthermore, it was suggested that the chitin-binding domain in RSC-a assists the antifungal action of RSC-a by binding to the fungal hypha.

• 社団法人日本農芸化学会の論文
• 2002-05-23

著者

• Yanagita T

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• ASO yoichi

  Laboratory of Protein Chemistry & Engineering, Kyushu University

• ISHIGURO Masatsune

  Laboratory of Protein Chemistry and Engineering, Kyushu University Graduate School of Bioresource an

• Yanagita Teruyoshi

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• Aso Y

  Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U

• Ohnuma Takeshi

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci

• YAMAGAMI Takeshi

  Laboratories of Protein Chemistry and Engineering

• OHNUMA Takayuki

  Laboratories of Protein Chemistry and Engineering

• Ishiguro M

  Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University

• TAIRA Toki

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University

• ISHIHARA Masanobu

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University

• Ishihara M

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci

• Ishihara Masanobu

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University

• Ishiguro Masatsune

  Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Facultr

• Yamagami T

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci

• Taira Toki

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University

• Taira T

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University

• Ishiguro Masatsune

  Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University

• ASO Yoichi

  Laboratory of Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University

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