Complete Amino Acid Sequences of Chitinase-1 and -2 from Bulbs of Genus Tulipa
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概要
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The complete amino acid sequences of tulip bulb chitinase-1 and -2 (TBC-1 and -2) were determined. The sequences of the TBC-1 and TBC-2 were solved by analysis of peptides derived by enzymatic digestions as well as by chemical cleavages with cyanogen bromide (CNBr), o-iodosobenzoic acid, and hydroxylamine. TBC-1 and TBC-2 both consisted of 275 amino acid residues and had molecular masses of 30,825 and 30,863,respectively. They shared 247 identical residues, that is 90% identity. Comparison of their sequences with that of gladiolus bulb class IIIb chitinase-a (GBC-a) showed that 63% of the residues of both TBC-1 and TBC-2 are identical to that of GBC-a. From these results, it was seen that TBC-1 and -2 are class IIIb chitinases. The characteristic difference in specific activity between TBC-1 and -2 was also discussed on the basis of their amino acid sequences.
- 社団法人日本農芸化学会の論文
- 1998-06-23
著者
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YAMAGAMI Takeshi
Laboratories of Protein Chemistry and Engineering
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Ishiguro Masatsune
Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University
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Ishiguro Masatsune
Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Facultr
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Yamagami Takeshi
Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University
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Ishiguro Masatsune
Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University
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