Purification and Some Properties of Three Chitinases from the Seeds of Rye (Secale cereale)

スポンサーリンク

概要

• 論文の詳細を見る

• Three chitinases, designated RSC-a, -b, and -c, were purified from the seeds of rye (Secale cereal) using ammonium sulfate precipitation, CM-cellulose column chromatography, gel filtration on Sephadex G-75, and S-Sepharose column chromatography. RSC-a, -b, and -c are basic proteins having molecular masses of 33 kDa, 26 kDa, and 26 kDa, and isoelectric points of 9.7, 10, and > 10, respectively. RSC-b and -c were found to be homologous proteins having similar amino acid compositions and N-terminal sequences. RSC-a contains more Thr, Ser, Glu, Pro, Gly, and Cys than RSC-b and -c and has a different N-terminal sequence from them. They hydrolyze glycolchitin and colloidal chitin, but not cell walls of Micrococcus lysodeikticus. These enzymes are stable at pH 4-8 and their optimum pHs toward glycolchitin are 5.

• 社団法人日本農芸化学会の論文
• 1993-04-23

著者

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• YAMAGAMI Takeshi

  Laboratories of Protein Chemistry and Engineering

• Yamagami Takeshi

  Laboratory Of Protein Chemistry & Engineering Faculty Of Agriculture Kyushu University

• Funatsu Gunki

  Laboratory Of Protein Chemistry & Engineering Faculty Of Agriculture Kyushu University

関連論文

• Some Properties of Ricin D Modified with a Methoxypolyethylene Glycol Derivative(Biological Chemistry)
• Further Studies on the Histidine Residue Located at the High Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
• Identification of the Tryptophan Residue Present in the Low Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
• Significance of the Histidine Residue at the High Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
• Significance of the Tryptophan Residue at the Low Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
• Chemical Modification of Ricin D with N-Acetylimidazole(Biological Chemistry)
• The Interaction of Castor Bean Lectins with Galactomannan(Biological Chemistry)
• Denaturation of Ricin D in Frozen Aqueous Solutions(Biological Chemistry)
• Base Specificity of Two Plant Seed Ribonucleases from Momoridica charantia and Luffa cylindrica
• Brefeldin A Protects Ricin-Induced Cytotoxicity in Human Cancer KB Cell Line, but not in its Resistant Counterpart with Altered Golgi Structures
• Primary Structure of 6.5k-Arginine/Glutamate-rich Polypeptide from the Seeds of Sponge Gourd (Luffa cylindrica)
• Chemical Modifications of Momordin-a and Luffin-a, Ribosome-Inactivating Proteins from the Seeds of Momordica charantia and Luffa cylindrica : Involvement of His140,Tyr165,and Lys231 in the Protein-Synthesis Inhibitory Activity
• Amino Acid Sequences of the Two Smallest Trypsin Inhibitors from Sponge Gourd Seeds(Biological Chemistry)
• Isolation and Amino Acid Sequence of a Protein-synthesis Inhibitor from the Seeds of Rye (Secale cereale)
• Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity of a Family 18 Chitinase from Tulip Bulbs(Biochemistry & Molecular Biology)
• Purification and Characterization of Three Mitogenic Lectins from the Roots of Pokeweed : (Phytolacca americana)
• Complete Amino Acid Sequence of Luffin-a, a Ribosome-inactivating Protein from the Seeds of Luff a cylindrica(Biological Chemistry)
• Complete Amino Acid Sequence of Luffin-b, a Ribosome-Inactivating Protein from Sponge Gourd (Luffa cylindrica) Seeds(Biological Chemistry)
• Structural Analysis of N-Linked Oligosaccharide of Mitogenic Lectin-B from the Roots of Pokeweed (Phytolacca americana)
• Purification of Chymotrypsin Inhibitors from Larval Hemolymph of the Silkworm, Bombyx mori
• Expression and Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity in a Ribonuclease MC1 from the Seeds of Bitter Gourd
• Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding Class I Chitinase from Rye (Secale cereale) Seeds
• Antifungal Activity of Rye (Secale cereale) Seed Chitinases : the Different Binding Manner of Class I and Class II Chitinases to the Fungal Cell Walls(Biochemistry & Molecular Biology)
• Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding Rye (Secale cereale) Seed Chitinase-c(Biochemistry & Molecular Biology)
• Localization, Accumulation, and Antifungal Activity of Chitinases in Rye (Secale cereale) Seed(Biochemistry & Molecular Biology)
• Isolation and Characterization of Chitinase Isoforms from the Bulbs of Four Species of the Genus Tulipa
• Complete Amino Acid Sequence of Chitinase-a from Bulbs of Gladiolus (Gladiolus gandavensis)
• Purification and Characterization of Two Chitinase Isoforms from the Bulbs of Gladiolus (Gladiolus gandavensis)
• The Amino Acid Sequence of Mitogenic Lectin-B from the Roots of Pokeweed : Phytolacca americana
• Amino Acid Sequence and Some Properties of Lectin-D from the Roots of Pokeweed (Phytolacca americana)
• The Complete Amino Acid Sequence of Lectin-C from the Roots of Pokeweed (Phytolacca americana)
• Role of the Histidine Residue at the Saccharide-binding Site of Castor Bean Hemagglutinin(Biological Chemistry)
• Chemical Modification of Castor Bean Hemagglutinin with Diethylpyrocarbonate(Biological Chemistry)
• States and Roles of Tryptophan Residues in Abrus precatorius Agglutinin(Biological Chemistry)
• Identification of the Tryptophan Residue Located at the Saccharide Binding Site of Castor Bean Hemagglutinin(Biological Chemistry)
• Purification and Characterization of Abrus Precatorius agglutinin
• Binding of Saccharides to Abrin-b Isolated from Abrus precatorius Seeds
• Chemical Modification of Tryptophan Residues in Abrin-a(Biological Chemistry)
• The Interaction of Abrin-a with Saccharides as Analyzed by Equilibrium Dialysis and Spectroscopy(Biological Chemistry)
• Spectroscopic Analysis of the Interaction of Ricin D with Urea(Biological Chemistry)
• Hydrophobicities of Ricin D and Its Constituent Polypeptide Chains(Biological Chemistry)
• Complete Amino Acid Sequence of Luffin-a, a Ribosome-inactivating Protein from the Seeds of Sponge Gourd (Luffa cylindrica)(Biological Chemistry)
• Isolation and Characterization of Two Momordins, Ribosome-Inactivating Proteins from the Seeds of Bitter Gourd (Momordica charantia)
• Partial Purification and Characterization of DOPA Quinone Imine Conversion Factor from Larval Hemolymph of the Silkworm, Bombyx mori
• Modification of Tryptophan Residues of Plant Class III Chitinases Involved in Enzyme Activity
• Positions of Disulfide Bonds in Rye (Secale cereale) Seed Chitinase-a
• Complete Amino Acid Sequence of Chitinase-A from Leaves of Pokeweed (Phytolacca americana)
• The Complete Amino Acid Sequence of Chitinase-B from the Leaves of Pokeweed (Phytolacca americana)
• Primary Structures of N-Linked Oligosaccharides of Momordin-a, a Ribosome-inactivating Protein from Momordica charantia Seeds(Biological Chemistry)
• Purification and Characterization of Two Chitinases from the Leaves of Pokeweed (Phytolacca americana)
• The Complete Amino Acid Sequences of the B-Chains of Abrin-a and Abrin-b, Toxic Proteins from the Seeds of Abrus precatorius
• Isolation of Glycopeptides from Ricinus communis Lectins(Biological Chemistry)
• Isolation and Characterization of Two Luffins, Protein-biosynthesis Inhibitory Proteins from the Seeds of Luffa cylindrica(Biological Chemistry)
• Involvement of C-Terminal Region in Inhibition of Protein Synthesis by Ricin A-Chain(Biological Chemistry)
• Purification and Characterization of the Constituent Polypeptide Chains of Abrus precatorius Agglutinin(Biological Chemistry)
• N-Acetyl-D-glucosamine-Asparagine Structure in Ribosome-inactivating Proteins from the Seeds of Luffa cylindrica and Phytolacca americana(Biological Chemistry)
• Isolation and Partial Characterization of Three Protein-synthesis Inhibitory Proteins from the Seeds of Luffa cylindrica(Biological Chemistry)
• The Amino Acid Sequence of Barley Rootlet Trypsin Inhibitor(Biological Chemistry)
• Significance of Carboxyl Group Present in the High Affinity Saccharide-binding Site of Ricin D(Biological Chemistry)
• Involvements of Trp23 in the Chitin-binding and of Trp131 in the Chitinase Activity of Rye Seed Chitinase-a
• Identification of the Tryptophan Residue Located at the Substrate-binding Site of Rye Seed Chitinase-c
• Effects of Chemical Modification of Arginine Residues Outside the Active Site Cleft of Ricin A-Chain on Its RNA N-Glycosidase Activity for Ribosomes
• Structural Analyses of Sugar Chains from Ricin A-Chain Variant(Biological Chemistry)
• Isolation and Amino Acid Sequences of Two Trypsin Inhibitors from the Seeds of Bitter Gourd (Momordica charantia)
• Isolation and Molecular Characterization of Four Arginine/Glutamate Rich Polypeptides from the Seeds of Sponge Gourd (Luffa cylindrica)
• Actions of Pokeweed Antiviral Protein on Virus-infected Protoplasts
• The Complete Amino Acid Sequence of the A-Chain of Abrin-a, a Toxic Protein from the Seeds of Abrus precatorius(Biological Chemistry)
• Isolation from α-Zein of Thermolysin Peptides with Angiotensin I-Converting Enzyme Inhibitory Activity
• Limited Proteolysis and Reduction-carboxymethylation of Rye Seed Chitinase-a: Role of the Chitin-binding Domain in Its Chitinase Action
• Effects of Salt Concentration on Translational Ability of Ricin A-Chain-treated Ribosomes(Biological Chemistry)
• The Complete Amino Acid Sequence of Momordin-a, a Ribosome-inactivating Protein from the Seeds of Bitter Gourd (Momordica charantia)
• Purification and Some Properties of a β-Xylosidase and an α-Fucosidase from Apple Snails (Pomacea canaliculata)
• The Complete Amino Acid Sequence of Chitinase-a from the Seeds of Rye (Secale cereal)
• The Complete Amino Acid Sequence of Chitinase-c from the Seeds of Rye (Secale cereal)
• Involvement of the B Chain C-Terminal Region in the High-affinity Saccharide Binding Site of Ricin D(Biological Chemistry)
• Identification of the Aspartic Acid Residue Located at or near Substrate-binding Site of Rye Seed Chitinase-c
• Cloning, Sequencing, and Expression of the Tulip Bulb Chitinase-1 cDNA
• Purification and Some Properties of Three Chitinases from the Seeds of Rye (Secale cereale)
• Complete Amino Acid Sequences of Chitinase-1 and -2 from Bulbs of Genus Tulipa
• The Complete Amino Acid Sequence of Antiviral Protein from the Seeds of Pokeweed (Phytolacca americana)(Biological Chemistry)
• Binding of Lactose and Galactose to Native and lodinated Ricin D
• Identification of Tyrosyl Residue Present in the High-affinity Saccharide-binding Site of Ricin D
• Induction of Superoxide Production in Polymorphonuclear Leucocytes by Ricinus Lectins and Its Dependency on Lectin Valency
• Chemical Modification of Tryptophan Residues in Castor Bean Hemagglutinin

もっと見る 閉じる

スポンサーリンク