N-Acetyl-D-glucosamine-Asparagine Structure in Ribosome-inactivating Proteins from the Seeds of Luffa cylindrica and Phytolacca americana(Biological Chemistry)

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概要

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• Glycosylation-site-containing peptides were isolated from the proteolytic digests of luffin-a, luffin-b, PAP-S and CNBr-fragments of PAP-S by reverse-phase HPLC, and their amino acid compositions and sequences were analyzed. Six peptides were obtained from luffin-a, and three each from luffin-b and PAP-S. All of these peptides were negative toward the phenol-H_2SO_4 reaction and gave only N-acetyl-D-glucosamine in gas chromatography after methanolysis and reacetylation. Amount of N-acetyl- D-glucosamine in these peptides were determined as D-glucosamine to be approximately one mol per peptide by an amino acid analyzer after HCl hydrolysis. Based on these results we concluded that Asn residues at positions of 28, 33, 77, 84, 206, and 227 in luffin-a, of 2, 78, and 85 in luffin-b, and of 10, 44, and 255 in PAP-S were glycosylated with only GlcNAc, and contained one residue per site.

• 社団法人日本農芸化学会の論文
• 1991-05-23

著者

• ISLAM M.Rafiqul

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Kimura Yoshinobu

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University:(present Of

• Kimura Yoshinobu

  Laboratory Of Biochemistry Faculty Of Agriculture Kyusyu University:(present Office)laboratory Of Bi

• KUNG Sung-Sil

  Laboratory of Protein Chemistry and Engineering, Faculty of Agriculture, Kyushu University

• Islam M.rafiqul

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• Kung Sung-sil

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• Kimura Yoshinobu

  Laboratory Of Bioapplied Enzymology Department Of Bioresources Chemistry Faculty Of Agriculture Okay

• KUNG Sung-Sil

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

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