Isolation and Characterization of Two Luffins, Protein-biosynthesis Inhibitory Proteins from the Seeds of Luffa cylindrica(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Two protein-biosynthesis inhibitory proteins, luffin-a and -b, were isolated from the seeds of the sponge gourd (Luffa cylindricd). Luffins were extracted from defatted meal of seeds of the sponge gourd with water at pH 4.0 and purified by gel filtration through a Sephadex G-75 column followed by CM-cellulose column chromatography and FPLC with Mono S column. Luffin-a and -b were basic proteins with isoelectric points of about 10, with molecular masses of 28,000 Da and 29,000 Da, respectively, as judged by SDS-PAGE. Luffin-a and -b were found to be homologous proteins having similar amino acid compositions (except threonine and proline), and having N-terminal sequences of Asp-Val-Arg-Phe-Ser-Leu-Ser-Gly- and Ala-Asn-Val-Ser-Phe-Ser-Leu-Ser-Gly-, respectively. Inhibitory activities of luffin-a and -b on cell-free protein synthesis were approximately 5.5 and 1.5 fold, respectively, stronger than that of ricin A-chain.
- 社団法人日本農芸化学会の論文
- 1988-05-23
著者
-
Nishida Hiromi
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
-
Nishida Hiromi
Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric
-
Funatsu Gunki
Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric
-
KAMENOSONO Masahiro
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
-
Kamenosono Masahiro
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
関連論文
- Some Properties of Ricin D Modified with a Methoxypolyethylene Glycol Derivative(Biological Chemistry)
- Further Studies on the Histidine Residue Located at the High Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
- Identification of the Tryptophan Residue Present in the Low Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
- Significance of the Histidine Residue at the High Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
- Significance of the Tryptophan Residue at the Low Affinity Saccharide-binding Site of Ricin E(Biological Chemistry)
- Chemical Modification of Ricin D with N-Acetylimidazole(Biological Chemistry)
- The Interaction of Castor Bean Lectins with Galactomannan(Biological Chemistry)
- Denaturation of Ricin D in Frozen Aqueous Solutions(Biological Chemistry)
- Base Specificity of Two Plant Seed Ribonucleases from Momoridica charantia and Luffa cylindrica
- Brefeldin A Protects Ricin-Induced Cytotoxicity in Human Cancer KB Cell Line, but not in its Resistant Counterpart with Altered Golgi Structures
- Primary Structure of 6.5k-Arginine/Glutamate-rich Polypeptide from the Seeds of Sponge Gourd (Luffa cylindrica)
- Chemical Modifications of Momordin-a and Luffin-a, Ribosome-Inactivating Proteins from the Seeds of Momordica charantia and Luffa cylindrica : Involvement of His140,Tyr165,and Lys231 in the Protein-Synthesis Inhibitory Activity
- Amino Acid Sequences of the Two Smallest Trypsin Inhibitors from Sponge Gourd Seeds(Biological Chemistry)
- Isolation and Amino Acid Sequence of a Protein-synthesis Inhibitor from the Seeds of Rye (Secale cereale)
- Purification and Characterization of Three Mitogenic Lectins from the Roots of Pokeweed : (Phytolacca americana)
- Complete Amino Acid Sequence of Luffin-a, a Ribosome-inactivating Protein from the Seeds of Luff a cylindrica(Biological Chemistry)
- Complete Amino Acid Sequence of Luffin-b, a Ribosome-Inactivating Protein from Sponge Gourd (Luffa cylindrica) Seeds(Biological Chemistry)
- Structural Analysis of N-Linked Oligosaccharide of Mitogenic Lectin-B from the Roots of Pokeweed (Phytolacca americana)
- Purification of Chymotrypsin Inhibitors from Larval Hemolymph of the Silkworm, Bombyx mori
- Expression and Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity in a Ribonuclease MC1 from the Seeds of Bitter Gourd
- The Amino Acid Sequence of Mitogenic Lectin-B from the Roots of Pokeweed : Phytolacca americana
- Amino Acid Sequence and Some Properties of Lectin-D from the Roots of Pokeweed (Phytolacca americana)
- The Complete Amino Acid Sequence of Lectin-C from the Roots of Pokeweed (Phytolacca americana)
- Role of the Histidine Residue at the Saccharide-binding Site of Castor Bean Hemagglutinin(Biological Chemistry)
- Chemical Modification of Castor Bean Hemagglutinin with Diethylpyrocarbonate(Biological Chemistry)
- States and Roles of Tryptophan Residues in Abrus precatorius Agglutinin(Biological Chemistry)
- Identification of the Tryptophan Residue Located at the Saccharide Binding Site of Castor Bean Hemagglutinin(Biological Chemistry)
- Purification and Characterization of Abrus Precatorius agglutinin
- Binding of Saccharides to Abrin-b Isolated from Abrus precatorius Seeds
- Chemical Modification of Tryptophan Residues in Abrin-a(Biological Chemistry)
- The Interaction of Abrin-a with Saccharides as Analyzed by Equilibrium Dialysis and Spectroscopy(Biological Chemistry)
- Spectroscopic Analysis of the Interaction of Ricin D with Urea(Biological Chemistry)
- Hydrophobicities of Ricin D and Its Constituent Polypeptide Chains(Biological Chemistry)
- Complete Amino Acid Sequence of Luffin-a, a Ribosome-inactivating Protein from the Seeds of Sponge Gourd (Luffa cylindrica)(Biological Chemistry)
- Isolation and Characterization of Two Momordins, Ribosome-Inactivating Proteins from the Seeds of Bitter Gourd (Momordica charantia)
- Positions of Disulfide Bonds in Rye (Secale cereale) Seed Chitinase-a
- Complete Amino Acid Sequence of Chitinase-A from Leaves of Pokeweed (Phytolacca americana)
- The Complete Amino Acid Sequence of Chitinase-B from the Leaves of Pokeweed (Phytolacca americana)
- Primary Structures of N-Linked Oligosaccharides of Momordin-a, a Ribosome-inactivating Protein from Momordica charantia Seeds(Biological Chemistry)
- Purification and Characterization of Two Chitinases from the Leaves of Pokeweed (Phytolacca americana)
- The Complete Amino Acid Sequences of the B-Chains of Abrin-a and Abrin-b, Toxic Proteins from the Seeds of Abrus precatorius
- Isolation of Glycopeptides from Ricinus communis Lectins(Biological Chemistry)
- Isolation and Characterization of Two Luffins, Protein-biosynthesis Inhibitory Proteins from the Seeds of Luffa cylindrica(Biological Chemistry)
- Involvement of C-Terminal Region in Inhibition of Protein Synthesis by Ricin A-Chain(Biological Chemistry)
- Purification and Characterization of the Constituent Polypeptide Chains of Abrus precatorius Agglutinin(Biological Chemistry)
- N-Acetyl-D-glucosamine-Asparagine Structure in Ribosome-inactivating Proteins from the Seeds of Luffa cylindrica and Phytolacca americana(Biological Chemistry)
- Isolation and Partial Characterization of Three Protein-synthesis Inhibitory Proteins from the Seeds of Luffa cylindrica(Biological Chemistry)
- The Amino Acid Sequence of Barley Rootlet Trypsin Inhibitor(Biological Chemistry)
- Significance of Carboxyl Group Present in the High Affinity Saccharide-binding Site of Ricin D(Biological Chemistry)
- Involvements of Trp23 in the Chitin-binding and of Trp131 in the Chitinase Activity of Rye Seed Chitinase-a
- Identification of the Tryptophan Residue Located at the Substrate-binding Site of Rye Seed Chitinase-c
- Effects of Chemical Modification of Arginine Residues Outside the Active Site Cleft of Ricin A-Chain on Its RNA N-Glycosidase Activity for Ribosomes
- Structural Analyses of Sugar Chains from Ricin A-Chain Variant(Biological Chemistry)
- Isolation and Amino Acid Sequences of Two Trypsin Inhibitors from the Seeds of Bitter Gourd (Momordica charantia)
- Isolation and Molecular Characterization of Four Arginine/Glutamate Rich Polypeptides from the Seeds of Sponge Gourd (Luffa cylindrica)
- Actions of Pokeweed Antiviral Protein on Virus-infected Protoplasts
- The Complete Amino Acid Sequence of the A-Chain of Abrin-a, a Toxic Protein from the Seeds of Abrus precatorius(Biological Chemistry)
- Isolation from α-Zein of Thermolysin Peptides with Angiotensin I-Converting Enzyme Inhibitory Activity
- Limited Proteolysis and Reduction-carboxymethylation of Rye Seed Chitinase-a: Role of the Chitin-binding Domain in Its Chitinase Action
- Effects of Salt Concentration on Translational Ability of Ricin A-Chain-treated Ribosomes(Biological Chemistry)
- The Complete Amino Acid Sequence of Momordin-a, a Ribosome-inactivating Protein from the Seeds of Bitter Gourd (Momordica charantia)
- Purification and Some Properties of a β-Xylosidase and an α-Fucosidase from Apple Snails (Pomacea canaliculata)
- The Complete Amino Acid Sequence of Chitinase-a from the Seeds of Rye (Secale cereal)
- The Complete Amino Acid Sequence of Chitinase-c from the Seeds of Rye (Secale cereal)
- Involvement of the B Chain C-Terminal Region in the High-affinity Saccharide Binding Site of Ricin D(Biological Chemistry)
- Identification of the Aspartic Acid Residue Located at or near Substrate-binding Site of Rye Seed Chitinase-c
- Purification and Some Properties of Three Chitinases from the Seeds of Rye (Secale cereale)
- The Complete Amino Acid Sequence of Antiviral Protein from the Seeds of Pokeweed (Phytolacca americana)(Biological Chemistry)
- Binding of Lactose and Galactose to Native and lodinated Ricin D
- Identification of Tyrosyl Residue Present in the High-affinity Saccharide-binding Site of Ricin D
- Induction of Superoxide Production in Polymorphonuclear Leucocytes by Ricinus Lectins and Its Dependency on Lectin Valency
- Chemical Modification of Tryptophan Residues in Castor Bean Hemagglutinin