Chemical Modification of Ricin D with N-Acetylimidazole(Biological Chemistry)

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概要

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• Tyrosine residues in ricin D were modified with N-acetylimidazole and the saccharide binding properties of the resulting derivatives were examined. The cytoagglutinating activity of ricin D was not altered by acetylation of one tyrosine residue/mol, but decreased greatly upon modification of two tyrosine residues/mol. In the cytoagglutination test, only 6% residual activity was found in the derivative, 2-Ac-ricin D, in which 2.4 tyrosine residues/mol were acetylated. In the presence of lactose, however, one tyrosine residue/mol was protected from acetylation by N-acetylimidazole with a retention of the saccharide binding ability, suggesting the involvement of one tyrosine residue in the saccharide binding. Fluorescence and UV-difference spectroscopic data indicate that the binding ability of the low affinity saccharide-binding site (LA-site) of ricin D remained unchanged after modification of 2 tyrosine residues/mol. The affinity chromatography of the acetylated derivatives of ricin D on the lactamyl- and galactosamine-cellulofine columns suggests that 2-Ac-ricin D binds galactopyranosides only at the LA-site, but lacks the binding ability at the high affinity saccharide-binding site (HA-site). It is postulated that introduction of the bulky acetyl group into the hydroxyl group of the tyrosine residue at the HA-site of ricin D may sterically hinder the binding of saccharides.

• 社団法人日本農芸化学会の論文
• 1989-06-23

著者

• Hatakeyama T

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Hatakeyama Tomomitsu

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Hatakeyama Tomomitsu

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University:(present Address)dept. Of Applie

• YAMASAKI Nobuyuki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• FUNATSU Gunki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• KAJIWARA Jumboku

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• MORIMOTO Kenji

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Tomomitsu Hatakeyama

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Morimoto Kenji

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Kajiwara Jumboku

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur

• Yamasaki Nobuyuki

  Institute Of Biophysics Faculty Of Agriculture Kyushu University

• Yamasaki N

  Kyushu Univ. Fukuoka Jpn

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Yamasaki Nobuyuki

  Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University

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