Preparation and Immunological Characterization of β-Lactoglobulin-Amylose Conjugate
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概要
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β-Lactoglobulin (BLG), a major allergen of cow’s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. The conjugate was prepared by modifying BLG with AG-ONSu, and was purified in a Sephadex G-100 column. The analytical data for AG-BLG indicated that 10.5 moles of AG-ONSu, with a mean molecular weight of 2,800, was covalently attached to the amino groups of the BLG molecule. Conjugation with AG-ONSu greatly decreased the reactivity of BLG with anti-BLG polyclonal antibodies owing to its shielding action for epitopes on the protein’s surface. These findings suggest that AG-ONSu can be used advantageously to suppress the hypersensitivity mediated by IgG antibodies in milk allergy.
- 社団法人 日本農芸化学会の論文
- 2011-01-23
著者
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YAMASAKI Nobuyuki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Yamasaki N
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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NODAKE Yuichi
Laboratory of Biochemistry, Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nagasaki Int
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FUKUMOTO Shoichi
Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of
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FUKASAWA Masashi
Laboratory of Biochemistry, Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nagasaki Int
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SAKAKIBARA Ryuzo
Laboratory of Biochemistry, Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nagasaki Int
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Nodake Yuichi
Laboratory Of Biochemistry Department Of Pharmacy Faculty Of Pharmaceutical Sciences Nagasaki Intern
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Fukumoto Shoichi
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Sakakibara Ryuzo
Laboratory Of Biochemistry Department Of Pharmacy Faculty Of Pharmaceutical Sciences Nagasaki Intern
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Fukasawa Masashi
Laboratory Of Biochemistry Department Of Pharmacy Faculty Of Pharmaceutical Sciences Nagasaki Intern
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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