Binding of Saccharides to Abrin-b Isolated from Abrus precatorius Seeds
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概要
- 論文の詳細を見る
The nature of the binding of saccharides to arbin-b, a toxic lectin isolated from Abrus precatorius seeds, was studied by equilibrium dialysis and fluorescence spectroscopy. Equilibrium dialysis data indicate that abrin-b has two saccharide-binding sites, a high affinity site (HA-site) and a low affinity site (LA-site), to which both galactopyranosides and N-acetylgalactosamine can bind. With excitation at 290 nm, abrin-b displayed a fluorescence spectrum with an emission maximum at 345nm. Upon binding with specific saccharides, this spectrum shifted to a wavelength shorter by 5 nm, suggesting that saccharides bind to abrin-b in such a manner as to induce a change in the environment of the tryptophan residue or residues at or near the respective binding sites. From the variation of fluorescence at 320 nm with saccharide concentrations, the association constants for binding of saccharides to the respective sites were measured. The results suggest that the HA-site has a subsite favorable for saccharides having β-1, 4 linked galactopyranoside at the non-reducing end like lactose in addition to the galactose-recognition site, while the LA-site may not have such a subsite.
- 社団法人日本農芸化学会の論文
- 1993-09-23
著者
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Ohba Hideki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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YAMASAKI Nobuyuki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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FUNATSU Gunki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Yamasaki N
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Ohba H
Kyushu National Industrial Res. Inst. Saga Jpn
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Funatsu G
Kyushu Univ. Fukuoka Jpn
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Funatsu Gunki
Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric
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Eguchi Koichirou
Laboratory of Biochemistry, and Laboratory of Protein Chemistry and Engineering, Faculty of Agricult
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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Eguchi Koichirou
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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