Some Properties of Ricin D Modified with a Methoxypolyethylene Glycol Derivative(Biological Chemistry)

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概要

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• A derivative of methoxypolyethylene glycol (mPEG_2) was covalently attached to ricin D, and some properties of the resulting conjugate (mPEG_2-ricin D) were studied. The conjugate was prepared by modification of ricin D with the N-hydroxysuccinimide ester of mPEG_2 [Yamasaki et al., Agric. Biol. Chem., 52, 2125 (1989)], and purified by DEAE-cellulose chromatography, followed by gel filtration on a Sephadex G-75 column. Analytical data indicated that in the conjugate, 9 mol of mPEG_2 with an average molecular weight of 10,000 were covalently attached to the ricin D molecule. The cytoagglutinating activity of mPEG_2-ricin D for Sarcoma 180 ascites tumor cells was 11% of that of native ricin D. While ricin D was insolubilized in frozen acidic solution at -20℃ accompanied by the loss of the cytoagglutinating activity irrespective of the coexistence of free mPEG_2, the conjugate was not insolubilized in frozen acidic solution and its cytoagglutinating activity was completely retained. An acetylated derivative of ricin D in which 7 amino groups/mol were modified with acetic anhydride was insolubilized in frozen solution similar to native ricin D. The insusceptibility of mPEG_2-ricin D to freeze-insolubilization may be due to the covalent attachment of the amphipathic copolymer, mPEG_2, to the amino groups of ricin D, but not due to the decrease in the positive charge by modification of amino groups.

• 社団法人日本農芸化学会の論文
• 1990-10-23

著者

• Hatakeyama T

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Hatakeyama Tomomitsu

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Hatakeyama Tomomitsu

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University:(present Address)dept. Of Applie

• YAMASAKI Nobuyuki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• MATSUO Akira

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• FUNATSU Gunki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Tomomitsu Hatakeyama

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Matsuo A

  Kyushu Univ. Fukuoka

• Yamasaki Nobuyuki

  Institute Of Biophysics Faculty Of Agriculture Kyushu University

• Yamasaki N

  Kyushu Univ. Fukuoka Jpn

• Funatsu G

  Kyushu Univ. Fukuoka Jpn

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Matsuo Akira

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Yamasaki Nobuyuki

  Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University

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