Purification and Characterization of Two Lectins from the Sea Cucumber Stichopus japonicus
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概要
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Two Ca^<2+>-dependent lectins were purified from the sea cucumber Stichopus japonicus by affinity chromatography on lactosyl-Sepharose 4B and ion-exchange chromatography on Q-Sepharose. Their molecular masses were estimated to be 13 kDa (SJL-I) and 15 kDa (SJL-II) on SDS-PAGE. SJL-I agglutinated rabbit erythrocytes as well as human A, B, and O-type erythrocytes, but SJL-II agglutinated only rabbit erythrocytes. Hemagglutination by SJL-I was competitively inhibited by N-acetyl-D-galactosamine and galactose-containing carbohydrates. On the other hand, only lactose, melibiose, and raffinose gave weak inhibition of hemagglutination by SJL-II, suggesting that SJL-II may have high specificity for particular complex carbohydrate(s) on the surface of rabbit erythrocytes. SJL-II was activated at ten times lower Ca^<2+> concentration than SJL-I. Both lectins lost activity in acidic pH, while SJL-I appeared more stable down to pH 4.5.
- 社団法人日本農芸化学会の論文
- 1993-10-23
著者
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Hatakeyama T
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Hatakeyama Tomomitsu
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
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Hatakeyama Tomomitsu
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University:(present Address)dept. Of Applie
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YAMASAKI Nobuyuki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Tomomitsu Hatakeyama
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Himeshima Tomoharu
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Komatsu Akane
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Komatsu Akane
Department Of Citriculture National Institute Of Fruit Tree Science
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Komatsu Akane
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
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Himeshima Tomoharu
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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