The Interaction of Abrin-a with Saccharides as Analyzed by Equilibrium Dialysis and Spectroscopy(Biological Chemistry)
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概要
- 論文の詳細を見る
The saccharide-binding properties of abrin-a, a toxic lectin from seeds of Abrus precatorius, were studied by equilibrium dialysis and spectroscopy. Equilibrium dialysis data suggest that abrin-a has two saccharide-binding sites with different affinities for galactopyranosides, a high affinity site (HA-site) and a low affinity site (LA-site). The binding of galactopyranosides to abrin-a induced a shift of the fluorescence spectrum to a shorter wavelength by 5 nm and UV-difference spectra with maxima at 283 and 292 nm. Such spectroscopic changes, however, were not induced by binding with N-acetylgalactosamine and galactosamine, suggesting a difference in the binding mode or the site to bind between these saccharides and galactopyranosides. The association constants for binding of individual saccharides to abrin-a suggested that in the HA-site there may be a subsite that can interact with the glucopyranosyl moiety of the lactose molecule in addition to the galactose-recognition site, while the LA-site may not have such a subsite structure. Based on these results, we concluded that galactopyranosides bind to abrin-a in such a manner as to induce the change in the environment of the tryptophan residue or residues located at or near the respective binding sites.
- 社団法人日本農芸化学会の論文
- 1990-08-23
著者
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Ohba Hideki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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YAMASAKI Nobuyuki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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FUNATSU Gunki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Yamasaki N
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Ohba H
Kyushu National Industrial Res. Inst. Saga Jpn
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Funatsu G
Kyushu Univ. Fukuoka Jpn
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Funatsu Gunki
Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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