Isolation and Amino Acid Sequence of a Protein-synthesis Inhibitor from the Seeds of Rye (Secale cereale)

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概要

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• A protein-synthesis inhibitor, designated RPSI, was isolated from the seeds of rye (Secale cereale) using gel filtration and S-Sepharose column chromatography. RPSI is a basic protein with an isoelectric point of over 10,and the concentration of protein required for 50% inhibition of protein synthesis (IC_50) of purified RPSI was about tenfold the concentration of ricin A-chain. The complete amino acid sequence of RPSI was discoverd by analyzing the peptides and fragments obtained from the proteolytic digests and by the cyanogen bromide- and hydroxylamine cleavages of RPSI. RPSI consists of 280 amino acid residues and has a molecular weight of 30,171. RPSI has only 21% sequence identity with that of ricin A-chain, but all five amino acid residues involved in the active site of ricin A-chain are conserved in RPSI.

• 社団法人日本農芸化学会の論文
• 1998-06-23

著者

• FUNATSU Gunki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Minami Y

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• Yagi Fumio

  Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

• Minami Yuji

  Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

• Yamaguchi Ken-ichi

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• TADERA Kenjiro

  Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

• Minami Y

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Yagi F

  Kagoshima Univ. Kagoshima Jpn

• Yagi Fumio

  Department Of Behavioral Neuroscience Kochi Medical School

• Minami Yuji

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• YAMAGUCHI Kenichi

  Laboratory of Protein Chemistry and Engineering, Faculty of Agriculture, Kyushu University

• Takezaki K

  Department Of Biochemical Science And Technology

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Tadera Kenjiro

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Yamaguchi Kenichi

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• Tadera Kenjiro

  Department of Biochemical Science and Technology

• TADERA Kenjiro

  Department of Agricultural Chemistry, Faculty of Agriculture, Kagoshima University

• YAGI Fumio

  Department of Agricultural Chemistry, Faculty of Agriculture, Kagoshima University

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