Isolation and Some Properties of the Antimicrobial Peptide (Pa-AMP) from the Seeds of Pokeweed (Phytolacca americana).

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概要

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• An antimicrobial peptide, designated Pa-AMP, was purified by gel filtration on Sephadex G-75 followed by S-Sepharose, Cosmosil-SP, and reverse-phase HPLC from the seeds of pokeweed (Phytolacca americana). Pa-AMP is a basic peptide having an isoelectric point of over 10 and its extinction coefficient at 280 nm of 1% aqueous solution was 7.7. Pa-AMP has a molecular mass of 4 kDa and 3.4 kDa on tricine SDS-PAGE under nonreducing and reducing conditions, respectively. The N-terminal amino acid of Pa-AMP was blocked. The concentrations of peptide required for 50% inhibition (IC_<50>) of the growth of plant pathogenic fungi, Gram-positive, and Gram-negative bacteria were 3 to 41 μg/ml. Differing from other peptides, Pa-AMP inhibited the growth of some Gram-negative bacteria.

• 社団法人日本農芸化学会の論文
• 1998-10-23

著者

• Minami Y

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• Yagi Fumio

  Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

• Minami Yuji

  Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

• TADERA Kenjiro

  Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

• Minami Y

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Yagi F

  Kagoshima Univ. Kagoshima Jpn

• Yagi Fumio

  Department Of Behavioral Neuroscience Kochi Medical School

• Minami Yuji

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• HIGUCHI Shinichiro

  Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

• Takezaki K

  Department Of Biochemical Science And Technology

• Tadera Kenjiro

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Higuchi Shinichiro

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Tadera Kenjiro

  Department of Biochemical Science and Technology

• TADERA Kenjiro

  Department of Agricultural Chemistry, Faculty of Agriculture, Kagoshima University

• YAGI Fumio

  Department of Agricultural Chemistry, Faculty of Agriculture, Kagoshima University

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