Purification and Characterization of a β-Glucosidase from Polygonum tinctorium, Which Catalyzes Preferentially the Hydrolysis of Indican

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概要

• 論文の詳細を見る

• An enzyme, β-glucosidase, which hydrolyzes indican (indoxyl-β-D-glucoside) was isolated in pure form from a plant, Polygonum tinctorium, and characterized. The enzyme showed specifically a low K_m for indican (0.34mM). The enzyme reacted with some other β-glucosides having aromatic groups but not with α-glucoside or other glycosides like galactoside. The activity was inhibited by several metal cations such as Cu^<2+> and by typical inhibitors for β-glucosidase such as D-glucono-1,5-lactone although these inhibitors required high concentrations.

• 社団法人日本農芸化学会の論文
• 1996-01-23

著者

• Minami Y

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• MIURA Kin-ichiro

  Institute for Biomolecular Science, Gakushuin University

• Miura Kazuo

  Department Of Biochemistry Okayama University Of Science

• Kanafuji Takeo

  Department of Biochemistry, Okayama University of science

• MINAMI Yoshiko

  Okayama university of Science, Faculty of Science, Department of Biochemistry

• KANAFUJI Takeo

  Okayama university of Science, Faculty of Science, Department of Biochemistry

• MIURA Kazuo

  Okayama university of Science, Faculty of Science, Department of Biochemistry

• Miura K

  National Inst. Animal Health Ibaraki Jpn

• Kanafuji Takeo

  Department Of Biochemistry Okayama University Of Science

• Miura K

  Gakushuin Univ. Tokyo

• Minami Y

  Okayama Univ. Sci. Okayama

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