High Performance in Refolding of Streptomyces griseus Trypsin by the Aid of a Mutant of Streptomyces Subtilisin Inhibitor Designed as Trypsin Inhibitor
スポンサーリンク
概要
- 論文の詳細を見る
- Japanese Biochemical Societyの論文
- 1999-02-01
著者
-
野原 大輔
名古屋市立大学薬学部 薬品製造工学教室
-
酒井 朝也
名古屋市立大学薬学部 薬品製造工学教室
-
NOHARA Daisuke
Faculty of Pharmaceutical Sciences, Nagoya City University
-
SUGIURA Hiroyuki
Faculty of Pharmaceutical Sciences, Nagoya City University
-
SAKAKIBARA Hiromu
Faculty of Pharmaceutical Sciences, Nagoya City University
-
MTSUBARA Mamoru
Faculty of Pharmaceutical Sciences, Nagoya City University
-
KOJIMA Shuichi
Institute for Biomolecular Science, Gakushuin University
-
MIURA Kin-ichiro
Institute for Biomolecular Science, Gakushuin University
-
SAKAI Tomoya
Faculty of Pharmaceutical Sciences, Nagoya City University
-
Miura Kazuo
Department Of Biochemistry Okayama University Of Science
-
Sakai Tomoya
Faculty Of Pharmaceutical Sciences Nagoya City University
-
Kojima S
Gakushuin Univ. Tokyo Jpn
-
Kojima Shuichi
Institute For Biomolecular Science Faculty Of Science Gakushuin University
-
Mtsubara Mamoru
Faculty Of Pharmaceutical Sciences Nagoya City University
-
Sakakibara Hiromu
Faculty Of Pharmaceutical Sciences Nagoya City University
-
Koizumi S
Institute For Biomolecular Science Gakushuin University
-
Miura K
Gakushuin Univ. Tokyo
-
Miura Kin-ichiro
Institute For Biomolecular Science Gakushuin Univ.:research Institute Chiba Institute Of Technology.
-
Nohara Daisuke
Faculty Of Engineering Gifu University
-
Miura Kin-ichiro
Institute For Biomolecular Science Faculty Of Science Gakushuin University
関連論文
- Application of Combined Reagent Solution to the Oxidative Refolding of Recombinant Human Interleukin 6
- Disulfide Bond Formation in Refolding of Thermophilic Fungal Protein Disulfide Isomerase
- プロテアーゼ・リフォールディングへの固定化インヒビター適用による高い活性回復率と効率的な精製・回収の達成
- Media Selection for Refolding of Thermolysin by Use of Immobilized Preparation
- もののかたちの創作と造作
- 3PD054 デシルトリメチルアンモニウムブロマイドのミセル溶液のESI/MSによる測定
- 1043 固定化インヒビターを用いたプロテアーゼの連続約リフォールディングの試み
- Kinetic Study on Thermal Denaturation of Hen Egg-White Lysozyme Involving Precipitation
- High Performance in Refolding of Streptomyces griseus Trypsin by the Aid of a Mutant of Streptomyces Subtilisin Inhibitor Designed as Trypsin Inhibitor
- 固定化inhibitorを用いたプロテアーゼのrefolding
- リフォールディングメディア選定のための固定化酵素の調製
- 質量分析計によるミセル分子量測定の可能性
- 1245 球状タンパク質のリフォールディングメディア選定のための3つの手法
- 201 球状タンパク質のrefoldingに効果のあった数種の試薬の役割
- Design of Optimum Refolding Solution by Combination of Reagents Classified by Specific Function
- A New Method of Media Selection for Protease Refolding by Application of Immobilized Subtilisin Preparation
- PREPARATION OF CHARACTERISTIC DIAGRAM FOR REFOLDING OF LYSOZYME
- 502 機能別に仕分けした試剤の組み合わせによる球状タンパク質のrefoldingにおけるタンパク質濃度の影響
- 501 タンパク質のrefolding media選定の新手法 : 固定化法の利用
- 734 特定作用能別に仕分けした試剤の組合せによる球状タンパク質の最適refolding溶液の設計
- Cloning and Expression of the Catalase Gene from the Anaerobic Bacterium Desulfovibrio vulgaris (Miyazaki F)
- β-Glucosidase in the Indigo Plant : Intracellular Localization and Tissue Specific Expression in Leaves
- Purification and Characterization of a β-Glucosidase from Polygonum tinctorium, Which Catalyzes Preferentially the Hydrolysis of Indican
- Translational Regulation of the mRNA Encoding the Eukaryotic Translation Initiation Factor 4E in Xenopus
- Binding Analysis of Xenopus laevis Translation Initiation Factor 4E (eIF4E) in Initiation Complex Formation
- Cloning and Expression of the Gene Encoding Flavodoxin from Desulfovibrio vulgaris(Miyazaki F)^1
- Primary Structure of Streptomyces griseus Metalloendopeptidase II
- 3PA020 固定化を利用したpepsinのin vitro refolding条件の選定
- 3PA019 Interluekin 6のV8 proteaseによる限定分解とその断片複合体の再構成
- 2Q19 野生型PDIとC末端欠損体のrefolding performanceの比較
- REFOLDING OF SUBTILISIN BPN' ACHIEVED ALMOST QUANTITATIVELY BY COVALENT IMMOBILIZATION ON AN AGAROSE GEL
- ESI-MSによるミセル分子量解析
- "Loose Folding" and "Delayed Oxidation" Procedures Successfully Applied for Refolding of Fully Reduced Hen Egg White Lysozyme
- DIFFERENCE BETWEEN GUANIDINIUM CHLORIDE AND UREA AS DENATURANTS OF GLOBULAR PROTEINS : THE POSSIBILITY OF APPLICATION TO IMPROVED REFOLDING PROCESSES
- アリルラジカルのアレンへの付加反応〔英文〕
- アリルラジカルのアレンへの付加に対する活性化エネルギ-〔英文〕
- Kinetic Anomalies in Chymotryptic Hydrolyses of p-Nitrophenyl Acetate and N-Benzoyl-L-alanine Methyl Ester
- Large ΔH^≠ and ΔS^≠ Values Obtained in Hydrolysis of α-Chymotrypsin or Catalase with α-Chymotrypsin
- アリルラジカルのプロピレンへの付加反応〔英文〕
- アリルラジカルのインデンへの付加反応〔英文〕
- アリルラジカルのメチルアセチレンへの環化的付加〔英文〕
- アリルラジカルとアセチレンの反応〔英文〕
- Structure-Activity Analysis of an Antimicrobial Peptide Derived from Bovine Apolipoprotein A-II
- In Vitro Refolding of Porcine Pepsin Immobilized on Agarose Beads
- Purification and Characterization of N, N-Dimethylformamidase from Alcaligenes sp. KUFA-1
- Analysis of the Isoform of Xenopus Euakryotic Translation Initiation Factor 4E
- Polysomes of eukaryotic cells observed by electron microscopy
- Transmission electron and atomic force microscopic observation of polysomes on carbon-coated grids prepared by surface spreading
- The Effects of the Side Chains of Hydrophobic Aliphatic Amino Acid Residues in an Amphipathic Polypeptide on the Formation of α Helix and Its Association
- Quantitative in vitro Renaturation of Subtilisin BPN' without the Aid of Pro-sequence
- High Frequency of SSI-Like Protease Inhibitors among Streptomyces
- 1P193 Application of polarized IR-microscope spectroscopy and solid-state NMR to structural analysis of protein fibers(6. Macromolecular assembly,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Accelerated Refolding of Subtilisin BPN' by Tertiary-Structure-Forming Mutants of Its Propeptide^1
- P1-Specificity of Aqualysin I (a Subtilisin-type Serine Protease) from Thermus aquaticus YT-1,using P1-Substituted Derivatives of Streptomyces Subtilisin Inhibitor
- Functional Tolerance of Streptomyces Subtilisin Inhibitor toward Conformational and Stability Changes Caused by Single-Point Mutations in the Hydrophobic Core
- 1P190 Toward creating metal binding helix fiber(6. Macromolecular assembly,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Codon and Base Biases after the Initiation Codon of the Open Reading Frames in the Escherichia coli Genome and Their Influence on the Translation Efficiency^1
- CYTOCHROME c OXIDASE ACTIVITY OF THE COPPERCONTAINING NITRITE REDUCTASE OF A PHOTOSYNTHETIC BACTERIUM
- A Subtilisin Inhibitor Produced by Streptomyces bikiniensis Possesses a Glutamine Residue at Reactive Site P1
- An Endogenous Target Protease, SAM-P26, of Streptomyces Protease Inhibitor(SSI): Primary Structure, Enzymatic Characterizaion, and Its Interaction with SSI
- Fibril Formation by an Amphipathic α-Helix-Forming Polypeptide Produced by Gene Engineering
- The Effect of Hairpin DNA Fragments on Escherichia coli Poly(U)-Dependent Poly(Phe)Synthesis
- Effects of γ -irradiation on E. coil harboring the pNVR-l planild with Ice Nucleation Active Gene
- The Multiplicity of N-Glycan Structures of Bovine Milk 18kDa Lactophorin (Milk GlyCAM-1)
- Inhibition of Translation and Progesterone-induced Maturation of Xenopus Oocytes by Expressing the Amino-terminal Portion of the Eukaryotic Translation Initiation Factor 4G(Biochemistry & Molecular Biology)
- Interference with Interaction between Eukaryotic Translation Initiation Factor 4G and Poly(A)-Binding Protein in Xenopus Oocytes Leads to Inhibition of Polyadenylated mRNA Translation and Oocyte Maturation
- Requirement of Ala Residues at g Position in Heptad Sequence of α-Helix-forming Peptide for Formation of Fibrous Structure
- Structure-activity analysis of an antimicrobial peptide derived from bovine apolipoprotein A-II
- Introduction of Enzymatic Activity to .ALPHA.-Lactalbumin by Artificial Exon Shuffling.
- The oxidation products of tetracyanoferrate(II) chelates with optically active 1,2-diamines: Tetracyano(1,2-diamine)ferrate(III)s and tetracyano(1,2-diimine)ferrate(II)s.
- Isomerization of Heptanes over a Palladium–Zeolite Catalyst
- The conformational interconversion of 1,3-diamine chelate: Tetracyano (1,3-propanediamine) ferrate (III).
- Circular dichroism spectra of some transition metal complexes with (1R,2R)-1,2-cyclopentanediamine.
- Orientation-dependent 1H and 13C isotropic shifts of low-spin tetracyano(1,2-diamine)ferrates(III).
- Binding Analysis of Xenopus laevis Translation Initiation Factor 4E (eIF4E) in Initiation Complex Formation.
- Functional Tolerance of Streptomyces Subtilisin Inhibitor toward Conformational and Stability Changes Caused by Single-Point Mutations in the Hydrophobic Core.
- Codon and Base Biases after the Initiation Codon of the Open Reading Frames in the Escherichia coli Genome and Their Influence on the Translation Efficiency.