Disulfide Bond Formation in Refolding of Thermophilic Fungal Protein Disulfide Isomerase

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概要

• 論文の詳細を見る

• Disulfide bond formation in the refolding of thermophilic fungal protein disulfide isomerase(PDI)was investigated. It was revealed that(i)a disulfide bond buried inside the molecule is preferentially formed and contributes to the thermal stability and the isomerizing power of PDI, and(ii)formation of disulfide bonds in active sites located on the molecular surface causes deformation of the optimum conformation resulting in a decrease in the thermal stability.

• 社団法人日本生物工学会の論文
• 2001-06-25

著者

• 野原 大輔

  名古屋市立大学薬学部 薬品製造工学教室

• Kurimoto Eiji

  Graduate School of Pharmaceutical Sciences, Nagoya City University

• Asami Osamu

  Toyota Central Research & Development Labs

• Kato Koichi

  Graduate School of Pharmaceutical Sciences, Nagoya City University

• Asami O

  Toyota Central Res. & Dev. Labs. Inc. Aichi Jpn

• HARADA Takushi

  Graduate School of Pharmaceutical Sciences, Nagoya City University

• SAKAI Tomoya

  Graduate School of Pharmaceutical Sciences, Nagoya City University

• NOHARA Daisuke

  Graduate School of Pharmaceutical Sciences, Nagoya City University

• TOKUHIRO KENROU

  Toyota Central Research & Development Labs.Inc.

• 酒井 朝也

  名古屋市立大学薬学部 薬品製造工学教室

• Kato K

  Toho Univ. Chiba Jpn

• Kato K

  Grad. Sch. Pharm. Sci. Nagoya City Univ.:okazaki Inst. Integ. Biosci. Natl. Inst. Natl. Sci.

• 栗本 英治

  Grad. Sch. Pharm. Sci. Nagoya City Univ.

• Kato Koichi

  Graduate School Of Pharmaceutical Sciences Nagoya City University:institute For Molecular Science Na

• Kurimoto Eiji

  Grad. Sch. Pharm. Sci. Nagoya City Univ.

• Harada Takushi

  Graduate School Of Pharmaceutical Sciences Nagoya City University

• Tokuhiro Kenrou

  Toyota Central Research & Development Labs.inc.

• Kato Koichi

  Graduate School Of Natural Sciences Nagoya City University

• Kato Koichi

  Okazaki Institute For Integrative Bioscience Institute For Molecular Science

• Kurimoto Eiji

  Graduate School Of Pharmaceutical Sciences Nagoya City University

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