A New Method of Media Selection for Protease Refolding by Application of Immobilized Subtilisin Preparation
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概要
- 論文の詳細を見る
In order to investigate the availability of the method using immobilized protein for selection of protein refolding media, two different types of immobilized subtilisin BPN' were prepared. One was immobilized on agarose gel by the amino groups of the enzyme and the other was immobilized on agarose gel by the carboxyl groups. Using the quantitatively renaturing preparations obtained by repeated denaturation/renaturation treatment of the immobilized subtilisin BPN', evaluation of various refolding media revealed that the two preparations exhibited similar trends with respect to the refolding yields induced by various media employed and that both of the immobilized subtilisin BPN' preparations were useful for media selection. Since immobilization can prevent interaction among protein molecules, we propose a selection method of refolding media, especially for refolding of protease, by which media minimizing misfolding are chosen with the immobilized enzyme preparation and the optimal medium for minimizing aggregation and autolysis is chosen from among them with the dissolved enzyme.
- 社団法人日本生物工学会の論文
- 1996-09-25
著者
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野原 大輔
名古屋市立大学薬学部 薬品製造工学教室
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HAYASHI Tetsuya
Department of Internal Medicine III, Osaka Medical College
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酒井 朝也
名古屋市立大学薬学部 薬品製造工学教室
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NOHARA DAISUKE
Department of Chemical Reaction Engineering, Faculty of Pharmaceutical Sciences, Nagoya City Univers
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SAKAI TOMOYA
Department of Chemical Reaction Engineering, Faculty of Pharmaceutical Sciences, Nagoya City Univers
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SUGIURA Hiroyuki
Faculty of Pharmaceutical Sciences, Nagoya City University
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SAKAKIBARA HIROMU
Department of Chemical Reaction Engineering, Faculty of Pharmaceutical Sciences, Nagoya City Univers
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KURIMOTO EIJI
Department of Chemical Reaction Engineering, Faculty of Pharmaceutical Sciences, Nagoya City Univers
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栗本 英治
Grad. Sch. Pharm. Sci. Nagoya City Univ.
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Sakai Tomoya
Department Of Chemical Reaction Engineering Faculty Of Pharmaceutical Sciences Nagoya City Universit
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Kurimoto Eiji
Grad. Sch. Pharm. Sci. Nagoya City Univ.
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Kurimoto Eiji
Department Of Applied Physics Osaka University
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Nohara Daisuke
Department Of Biomolecular Science Faculty Of Engineering Gifu University
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Hayashi Tetsuya
Department Of Chemical Reaction Engineering Faculty Of Pharmaceutical Sciences Nagoya City Universit
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Hayashi Tetsuya
Department Of Bacteriology School Of Medicine Shinsu University
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Sakakibara Hiromu
Faculty Of Pharmaceutical Sciences Nagoya City University
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